NUTRITIONAL INTAKE AND PROINFLAMMATORY CYTOKINE ACTIVITY IN COMMUNITY DWELLING S

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。促炎细胞因子在慢性病患者和健康老年人中升高。这些细胞因子是否对健康成年人的营养摄入具有与慢性疾病相同的负面影响尚不清楚。这项研究的目的是检查一组健康老年人的营养摄入和促炎细胞因子活性之间的关系。本研究将招募40名年龄在65岁或以上且无慢性病的社区居住成年人。将使用4天食物日记评估营养摄入量。将抽取血液并分析血清中的TNF？，可溶性TNF受体sTNFR 1和sTNFR 2、IL-1 ra、IL-2、IL-4、IL-6、IL-8、IL-10、内毒素、白蛋白、CRP、尿酸和脂质组。将采集24小时尿液并分析钠和氮。将使用每人营养缺乏总数的描述性检查来描述该组的营养摄入量。炎症标志物和营养摄入之间的相互作用将通过比较两组之间的卡路里摄入、蛋白质摄入和营养缺乏的数量除以血清细胞因子水平的中位数来确定。膳食脂肪酸摄入对促炎细胞因子活性的影响将通过比较两组之间的促炎细胞因子水平除以三种脂肪酸的中位摄入来确定。这项研究将提供数据，比较我们的心力衰竭患者的脂肪酸对细胞因子活性的影响，以及细胞因子是否参与了与正常衰老和心力衰竭相关的食物摄入减少。这些信息将有助于制定适当的干预措施，以根据老年人的健康状况改善和维持老年人的营养摄入。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proinflammatory cytokines are elevated in people with chronic illness and in healthy older adults. Whether these cytokines have the same negative effect on nutrient intake in healthy adults as in chronic illness is unknown. The purpose of the proposed research is to examine the relationships between nutritional intake and proinflammatory cytokine activity in a group of healthy older adults. Forty community-dwelling adults aged 65 years or older and free of chronic illness will be recruited for the study. Nutritional intake will be assessed using four-day food diaries. Blood will be drawn and serum analyzed for TNF?, soluble TNF receptors sTNFR1 and sTNFR2, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, endotoxin, albumin, CRP, uric acid, and lipid panel. A 24-hour urine will be collected and analyzed for sodium and nitrogen. A descriptive examination of total number of nutrient deficiencies per person will be used to describe the nutritional intake of the group. The interaction among inflammatory markers and nutrtional intake will be determined by comparing calorie intake, protein intake, and number of nutritional deficiencies between two groups divided by a median split of serum cytokine levels. The effect of dietary fatty acid intake on proinflammatory cytokine activity will be determine by comparing proinflammatory cytokine levels between two groups divided by median intake of three fatty acids. This study will provide data for comparison with our heart failure patients with respect to the effects of fatty acids on cytokine activity and whether cytokines are invovled in both the decrease in food intake associated with normal aging and heart failure. This information will assist in developing appropriate interventions to improve and maintain nutritional intake of older adults according to their health status.