EVALUATION OF AN OMPP2A OMPP2B DOUBLE MUTANT IN EXPERIMENTAL HUMAN INFECTION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。杜克雷嗜血杆菌是生殖器溃疡性疾病软下疳的病原体。两个H ducreyi蛋白，命名为OmpP 2A和OmpP 2B，与其它主要孔蛋白具有同源性。孔蛋白具有重要的抗原决定簇，提供膜稳定性，并影响抗生素敏感性（1-3）。孔蛋白是人杀菌抗体的靶标（4）。本研究的目的是检验OmpP 2A OmpP 2B双突变体与其同基因亲本相比感染人皮肤的能力受损的假设。为了验证这一假设，我们将比较亲本和突变体在人类受试者中引起实验性感染的能力。这些研究将确定OmpP 2A和OmpP 2B的表达是否是H. ducreyi引起脓疱，并可能提供一个理论基础，开发疫苗，针对H。杜克雷

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Haemophilus ducreyi is the etiologic agent of the genital ulcer disease chancroid. Two H. ducreyi proteins, named OmpP2A and OmpP2B, have homology with other major porin proteins. Porins bear important antigenic determinants, provide membrane stability, and influence antibiotic susceptibility (1-3). Porins are targets of human bactericidal antibodies (4). The purpose of this study is to test the hypothesis that an OmpP2A OmpP2B double mutant is impaired in its ability to infect human skin when compared to its isogenic parent. To test this hypothesis, we will compare the ability of the parent and the mutant to cause experimental infection in human subjects. These studies will establish whether expression of OmpP2A and OmpP2B is required for H. ducreyi to cause pustules, and may provide a rationale to develop a vaccine against H. ducrey