MACROPHAGE RESPONSE TO LPS IN PERIODONTAL DISEASE AND DIABETES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这项NIH资助的研究是基于巨噬细胞对细菌脂多糖（LPS）的反应通过提高促炎细胞因子（如TNF-a、il - 1b和PGE2）的产生介导牙周病的进展和严重程度的前提。该研究特别关注糖尿病患者，因为在这些患者中牙周病非常严重，并且与牙周病进展相关的细胞因子反应已被证明在糖尿病患者中明显高于非糖尿病患者。200名患者，年龄21-65岁，患有糖尿病，有或没有标准标准定义的牙周病，将被招募到研究中。将从研究患者那里获得有关医疗和牙科病史以及药物的背景人口统计信息。一次性抽取每位患者50 mL静脉血样本，从这些样本中分离单核细胞和LPS，用于吞噬细胞活化和LPS刺激单核细胞/巨噬细胞的实验室研究。此外，该血样的一部分将用于IL-1基因簇多态性的遗传研究，以确定多态性与糖尿病患者牙周炎发生之间的关系，如果有的话。最后，将从牙周袋中获得细菌和牙周液样本，以表征患者牙周组织中存在的微生物并测量细胞因子水平

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This NIH funded study is based on the premise that macrophage responses to bacterial lipopolysaccharides (LPS) mediate the progression and severity of periodontal disease through elevated production of pro-inflammatory cytokines (e.g. TNF-a, IL1b, and PGE2). The study focuses in particular on diabetic patients because periodontal disease is excessive among these patients, and cytokine responses associated with progression of periodontal disease have been shown to be markedly higher in diabetic patients than those observed in non-diabetic individuals. 200 patients, ages 21-65, with diabetes, with and without periodontal disease defined by standard criteria, will be recruited to the study. Background demographic information concerning medical and dental histories and medications will be obtained from study patients. One-time, 50 mL venous blood samples will be drawn from each patient, and monocytes and LPS will be isolated from these samples for laboratory studies of phagocytic cell activation and LPS stimulation of monocyte/macrophages. In addition, portions of this blood sample will be used for genetic studies of polymorphisms in the IL-1 gene cluster for the sake of identifying relationships, if any, between polymorphisms and the occurrence of periodontitis in diabetic individuals. Finally, bacteria and periodontal fluid samples will be obtained from periodontal pockets to characterize the microorganisms present in the periodontal tissues of patients and to measure cytokine levels at the