HYDROXYPROLIINE AND URINARY OXALATE EXCRETION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。当胶原蛋白和明胶被食用时，它们会在体内部分分解，产生小的有机酸。这些酸包括甘氨酸、乙醛酸盐、乙醇酸盐和草酸盐。草酸盐是肾结石的主要成分，其合成与病理有关。在一些患有遗传性疾病的个体中，羟脯氨酸的代谢可能受损，并且可能形成过量的草酸盐。在这个项目中，明胶对这些有机酸的代谢将在正常个体中进行测量。个人将食用含有明胶或鸡蛋蛋白的已知成分的饮食。有机酸的形成将在血液和尿液样本中测量。这项研究应该揭示胶原蛋白或明胶的分解是否与正常个体的草酸形成有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. When collagen and gelatin are eaten, they are broken down in the body in part to yield small organic acids. These acids include glycine, glyoxylate, glycolate, and oxalate. Oxalate is a major component of kidney stones and its synthesis is of pathologic concern. In some individuals with genetic diseases, the metabolism of hydroxyproline may be impaired, and excessive amounts of oxalate may be formed. In this project, the metabolism of gelatin to these organic acids will be measured in normal individuals. Individuals will consume diets of known composition that contain either gelatin or egg protein as part of the diet. The formation of organic acids will be measured in blood and urine samples. This study should reveal whether collagen or gelatin breakdown is associated with oxalate formation in normal individuals.