HPV INFECTION AND HIGH FRUIT AND VEGETABLE DIET: PILOT PROJECT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究的具体目标是：(1)估计感染人乳头状瘤病毒(HPV)的妇女将改变饮食以包括每天七份或更多营养丰富的水果和蔬菜的比例，(2)估计在饮食干预之前、期间和之后血清中类胡萝卜素、胆固醇、生育激素、视黄醇和叶酸的浓度，并将这些浓度与HPV感染妇女的饮食自我报告相关联，(3)验证风险评估问卷的可行性和有效性，(4)评估宫颈HPV感染的持续性，以及(5)估计被筛选的潜在参与者被投入研究的比例。为了实现这一目标，30名感染HPV的女性将被招募来坚持每天7份营养丰富的水果和蔬菜的研究饮食。每两个月，参与者将接受宫颈HPV感染测试，抽取一汤匙血液，并回答健康和行为问卷。每个参与者将被跟踪总共6个月。在6个月的时间里，参与者将与注册营养师进行四次面对面的咨询会议，并每两周进行一次电话或电子邮件后续对话。类胡萝卜素、胆固醇、生育酚、视黄醇和叶酸的血清将在基线上提取。类胡萝卜素和胆固醇的血清将在第2、4和6个月重复进行。膳食摄入量将在基线和第6个月使用弗雷德·哈钦森食物频率问卷进行测量。感兴趣的主要结果将是每两个月出现HPV(致癌和非致癌类型)的次数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific objectives for this study are: (1) to estimate the portion of women with human papillomavirus (HPV) who will modify their diet to include seven or more servings of nutrient dense fruits and vegetables each day, (2) to estimate serum concentration of carotenoids, cholesterol, tocopherois, retinol, and folate before, during, and after the dietary intervention, and correlate these concentrations with self-reports of diet among women with HPV, (3) to verify the feasibility and validity of the risk assessment questionnaire, (4) to assess the persistence of cervical HPV infection, and (5) to estimate the proportion of screened potential participants that are put on study. To accomplish this aim, 30 women who have HPV will be recruited to adhere to a study diet of > 7 servings of nutrient dense fruits and vegetables per day. Every two months, participants will be tested for HPV infection from the cervix, have a tablespoon of blood drawn and respond to health and behavior questionnaires. Each participant will be followed for a total of 6 months. During the 6 month period, participants will have four in-person counseling sessions with a registered dietician and biweekly phone or email follow-up conversations. Serum for carotenoids, cholesterol, tocopherols, retinol, and folate will be drawn at baseline. The serum for carotenoids and cholesterol will be repeated at months 2, 4, and 6. Dietary intake will be measured using the Fred Hutchinson Food Frequency Questionnaire at baseline and month 6. The primary outcome of interest will be number of every two month visits with HPV present (oncogenic and nonocogenic type).