AIRFLOW OBSTRUCTION AND BIOMARKERS OF AIRWAY INFLAMATION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本研究是一项纵向单中心试点研究，旨在描述6-18岁儿童因哮喘住院一个月后肺功能和气道炎症非侵入性生物标志物水平的变化。在儿童医院和地区医疗中心（GCRC）因哮喘加重入院的20名6-18岁哮喘儿童将被纳入研究，并在出院前完成初步研究访问。在首次就诊期间，受试者将接受临床评估并进行肺活量测定来测量肺功能。此外，在20分钟的潮汐呼吸过程中，将测量呼出的一氧化氮（eNO）浓度，并收集呼出冷凝水（eBC）样本。呼气冷凝水将被分析以确定半胱氨酸白三烯（CysLT）的浓度，这是哮喘气道炎症的重要介质。哮喘患者将在出院后1周、2周和4周返回CHRMC的GCRC儿科卫星进行三次随访研究访问。在随访期间，研究者或GCRC注册护士将在研究访问之间对症状和药物使用情况进行问卷调查。此外，受试者将进行肺活量测定，测量eNO浓度，并收集呼出液以测量CysLT浓度。哮喘患者将在出院后1周、2周和4周返回CHRMC的GCRC儿科卫星进行3次随访研究访问。在随访期间，受试者将完成一份关于自最近一次研究访问以来的症状和药物使用情况的问卷，将进行肺活量测定，并测量eNO浓度和收集呼出液用于CysLT分析。20名6-18岁无哮喘或过敏的健康对照受试者也将参与研究。对照受试者将在chmc的CGRC儿科卫星中心完成一次单独的研究访问。在研究访问期间，将进行人口统计和病史问卷调查，并进行体格检查。对照组受试者将完成肺活量测定，进行eNO测量，并在20分钟潮汐呼吸期间收集eBC用于CysLT分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study is a longitudinal single-center pilot study designed to describe changes in lung function and levels of noninvasive biomarkers of airway inflammation in children ages 6-18 years over one month following hospitalization for status asthmaticus. Twenty children ages 6-18 years with asthma who are admitted to Children's Hospital and Regional Medical Center (GCRC) for an asthma exacerbation will be enrolled and complete an initial study visit prior to hospital discharge. During their initial visit subjects will undergo a clinical assessment and perform spirometry to measure lung function. In addition, exhaled nitric oxide (eNO) concentration will be measured and a sample of exhaled breath condensate (eBC) will be collected during 20 minutes of tidal breathing. Breath condensate will be analyzed to determine the concentration of cysteinyl leukotrienes (CysLT), an important mediator of airway inflammation in asthma. Subjects with asthma will return to the GCRC pediatric satellite at CHRMC for three follow-up study visits, at 1 week, 2 weeks, and 4 weeks following hospital discharge. During follow-up visits an investigator or GCRC RN will administer a questionnaire regarding symptoms and medication use between study visit. In addition, subjects will perform spirometry, have eNO concentration measured, and have breath condensate collected to measure CysLT concentration. Subjects with asthma will return to the GCRC pediatric satellite at CHRMC for 3 follow-up study visits, at 1 week, 2 weeks, and 4 weeks following hospital discharge. During follow-up visits subjects will complete a questionnaire regarding symptoms and medication use since the most recent study visit, will perform spirometry, and have eNO concentration measured and breath condensate collected for CysLT analysis. Twenty healthy control subjects ages 6-18 years without asthma or allergies will also participate. Control subjects will complete a single study visit at the CGRC pediatric satellite at CHRMC. During the study visit a demographic and medical history questionnaire will be administered and a physical exam performed. Control subjects will complete spirometry, a measurement of eNO will be performed, and eBC collected during 20 minutes of tidal breathing for CysLT analysis.