AIRFLOW OBSTRUCTION AND BIOMARKERS OF AIRWAY DURING AND FOLLOWING OF ASTHMA

哮喘期间和之后的气流阻塞和气道生物标志物

• 批准号: 7603551
• 负责人: JASON S DEBLEY
• 金额: $ 0.22万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2007-09-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7603551
• 关键词: Age; Air; Asthma; Biological Markers; Breathing; Child; Childhood; Clinical assessments; Computer Retrieval of Information on Scientific Projects Database; Cotinine; Enrollment; Epithelial Cells; Exhalation; Funding; Grant; Hospitalization; Hypersensitivity; Institution; Measurement; Measures; Mediator of activation protein; Medical History; Nasal Lavage Fluid; Nicotine; Nitric Oxide; Obstruction; Pediatric Hospitals; Pharmaceutical Preparations; Pilot Projects; Polymerase Chain Reaction; Procedures; Questionnaires; Research; Research Design; Research Personnel; Resources; Respiratory physiology; Source; Spirometry; Status Asthmaticus; Symptoms; United States National Institutes of Health; Urine; Viral; Visit; Week; airway inflammation; cysteinyl-leukotriene; follow-up

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a longitudinal single-center pilot study designed to describe changes in lung function and levels of noninvasive biomarkers of airway inflammation in children ages 6-18 years over two months following hospitalization for status asthmaticus. Forty children ages 6-18 years with asthma, admitted to Children's Hospital (GCRC) for an asthma exacerbation will be enrolled, completing a study visit prior to discharge. Subjects will undergo a clinical assessment, perform spirometry to measure lung function, measure exhaled nitric oxide (eNO), collect exhaled breath condensate (eBC), collect urine for cotinine level (nicotine exposure, repeated at visit 4). Nasal wash and brushings for viral PCR and epithelial cell culture will also be done and repeated at visit 5. eBC will be analyzed for cysteinyl leukotrienes (CysLT), an important mediator of airway inflammation. These subjects will return to the GCRC for four follow-up visits over eight weeks. A questionnaire will be administered regarding symptoms and medication use between study visits. Subjects will repeat all procedures at visits 2-5. A cold, dry air challenge (to measure airway twitchiness) will be done at visit 4. Forty healthy control subjects ages 6-18 years without asthma or allergies will also participate. Control subjects will complete a single study visit at the CGRC pediatric satellite at CHRMC. During the study visit a demographic and medical history questionnaire will be administered and a physical exam performed. Control subjects will complete spirometry, a measurement of eNO will be performed, and eBC collected during 20 minutes of tidal breathing for CysLT analysis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这是一项纵向单中心试点研究，旨在描述 6-18 岁儿童因哮喘持续状态住院两个月后肺功能和气道炎症非侵入性生物标志物水平的变化。将招募 40 名因哮喘恶化而入住儿童医院 (GCRC) 的 6-18 岁哮喘儿童，并在出院前完成研究访视。受试者将接受临床评估、进行肺活量测定以测量肺功能、测量呼出一氧化氮 (eNO)、收集呼出气冷凝物 (eBC)、收集尿液测定可替宁水平（尼古丁暴露，在访视 4 时重复）。还将进行洗鼻和刷牙以进行病毒 PCR 和上皮细胞培养，并在第 5 次访视时重复。将分析 eBC 中的半胱氨酰白三烯 (CysLT)，这是气道炎症的重要介质。 这些受试者将返回 GCRC 在八周内进行四次随访。 在研究访问之间将进行有关症状和药物使用的调查问卷。 受试者将在第 2-5 次访视时重复所有程序。第 4 次访视时将进行冷、干燥空气挑战（以测量气道抽搐）。四十名年龄 6-18 岁、无哮喘或过敏的健康对照受试者也将参加。对照受试者将在 CHRMC 的 CGRC 儿科卫星上完成一次研究访问。在研究访问期间，将进行人口统计和病史调查问卷并进行体检。 对照受试者将完成肺量测定、eNO 测量，并在 20 分钟潮式呼吸期间收集 eBC 用于 CysLT 分析。