AIRFLOW OBSTRUCTION AND BIOMARKERS OF AIRWAY DURING AND FOLLOWING OF ASTHMA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这是一项纵向单中心初步研究，旨在描述6-18岁儿童因哮喘持续状态住院治疗后两个月内肺功能和气道炎症非侵入性生物标志物水平的变化。将入组40名6-18岁哮喘儿童，因哮喘急性发作入住儿童医院（GCRC），在出院前完成研究访视。受试者将接受临床评估，进行肺活量测定以测量肺功能，测量呼出的一氧化氮（eNO），收集呼出气冷凝液（eBC），收集尿液用于可替宁水平（尼古丁暴露，在访视4时重复）。访视5时还将进行鼻清洗和刷拭，以进行病毒PCR和上皮细胞培养。将分析eBC的半胱氨酰白三烯（CysLT），这是气道炎症的重要介质。 这些受试者将在8周内返回GCRC接受4次随访访视。 将在研究访视之间进行关于症状和药物使用的问卷调查。 受试者将在访视2-5时重复所有程序。将在访视4时进行寒冷干燥空气激发（以测量气道抽搐）。 40名6-18岁的健康对照受试者也将参加，他们没有哮喘或过敏。对照受试者将在CHRMC的CGRC儿科卫星完成单次研究访视。研究访视期间，将进行人口统计学和病史问卷调查，并进行体格检查。 对照受试者将完成肺量测定，测量eNO，并在20分钟潮气呼吸期间收集eBC用于CysLT分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a longitudinal single-center pilot study designed to describe changes in lung function and levels of noninvasive biomarkers of airway inflammation in children ages 6-18 years over two months following hospitalization for status asthmaticus. Forty children ages 6-18 years with asthma, admitted to Children's Hospital (GCRC) for an asthma exacerbation will be enrolled, completing a study visit prior to discharge. Subjects will undergo a clinical assessment, perform spirometry to measure lung function, measure exhaled nitric oxide (eNO), collect exhaled breath condensate (eBC), collect urine for cotinine level (nicotine exposure, repeated at visit 4). Nasal wash and brushings for viral PCR and epithelial cell culture will also be done and repeated at visit 5. eBC will be analyzed for cysteinyl leukotrienes (CysLT), an important mediator of airway inflammation. These subjects will return to the GCRC for four follow-up visits over eight weeks. A questionnaire will be administered regarding symptoms and medication use between study visits. Subjects will repeat all procedures at visits 2-5. A cold, dry air challenge (to measure airway twitchiness) will be done at visit 4. Forty healthy control subjects ages 6-18 years without asthma or allergies will also participate. Control subjects will complete a single study visit at the CGRC pediatric satellite at CHRMC. During the study visit a demographic and medical history questionnaire will be administered and a physical exam performed. Control subjects will complete spirometry, a measurement of eNO will be performed, and eBC collected during 20 minutes of tidal breathing for CysLT analysis.