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Clinical Impacts of Heterogeneous Interferon Responses to Viral Infection by Asthmatic Airway Epithelium

异质干扰素对哮喘气道上皮病毒感染反应的临床影响

基本信息

批准号：
10330561
负责人：
JASON S DEBLEY
金额：
$ 18.45万
依托单位：
SEATTLE CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-10 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10330561
关键词：
Adrenal Cortex Hormones Air Asthma Biological Models Biological Products Cell Culture Techniques Cell Line Cell model Cells Child Childhood Childhood Asthma Clinical Code Emergency department visit Environment Epithelial Epithelial Cells Fibroblasts Frequencies Gene Expression Genes Genetic Polymorphism Goals Heterogeneity Hospitalization Human Incidence Infection Inflammation Inhalation Innate Immune Response Interferons Learning Liquid substance Lung Lung diseases Modeling Morbidity - disease rate Myofibroblast Natural History Nose Pathogenesis Patients Phenotype Play Primary Infection Regulation Reporting Research Research Personnel Research Technics Resources Respiratory Syncytial Virus Infections Rhinovirus Rhinovirus infection Risk Role Schools Series Signal Transduction Pathway Stromal Cells Subgroup Techniques Testing Time Training Translational Research Viral Virus Virus Diseases Virus Replication airway epithelium airway remodeling asthma exacerbation asthmatic asthmatic airway asthmatic patient base career cohort cost cytokine experimental study functional decline loss of function patient oriented patient oriented research prevent programs prospective pulmonary function pulmonary function decline response sensor translational study

项目摘要

Project Summary Asthma exacerbations among U.S children result in 640,000 emergency department visits and 14 million missed school days annually. Viral infections trigger the majority of exacerbations in children, of which human rhinoviruses (HRV) are the most common. The airway epithelium plays central roles in regulating inflammation, airway remodeling responses, and innate immune responses to infection. The most striking response of airway epithelial cells (AECs) to viral infection is expression of type I and III interferons (IFN I/III) and IFN stimulated genes (ISG). Some have reported deficient IFN I/III responses to viral infection by asthmatic AECs and postulated that deficient epithelial IFN responses to viruses predispose to exacerbations, whereas others have not observed differences in AEC IFN responses to viruses between asthmatic and healthy AECs. In our unique cohort of well characterized asthmatic and healthy children, from whom we obtain bronchial and nasal AECs and conduct mechanistic experiments using air-liquid-interface organotypic culture models, we have observed significant heterogeneity in IFN I/III responses to HRV and RSV infection. For example, among our asthmatic AEC donors we have noted associations between high type I/III IFN responses and lower donor lung function, as well as distinct subgroups of exacerbation prone asthmatics with deficient IFN I/III responses to HRV. Heterogeneity in AEC IFN I/III responses to viral infections may be explained by polymorphisms in genes coding for viral sensors and/or key steps in signal transduction pathways upstream of IFN I and III. The overall goal my research program is to understand how airway epithelial responses influence viral-triggered exacerbations and airway remodeling mechanisms in asthmatic children. In the first aim, using primary cells from children with asthma we will test our hypothesis that polymorphisms in genes coding for viral sensors, and/or key steps in signal transduction pathways upstream of IFN responses, contribute to heterogeneity in IFN I/III responses to HRV infection by AECs from asthmatic children. In the second aim, we will prospectively follow a cohort of asthmatic children to test our hypothesis that deficient AEC IFN I/III responses to HRV are associated with a greater incidence of viral-triggered exacerbations in AEC donors. In the final aim, we will test our hypothesis that excessively high IFN I/III responses by asthmatic AECs to HRV are associated with lung function decline among AEC donors, and we will interrogate potential mechanisms whereby excessively high IFN I/III responses may promote proliferation and activation of lung stromal cells. The studies conducted in my research program and unique resource of primary airway epithelial cells from clinically well characterized asthmatic children provide an exceptional training environment for young investigators dedicated to a career in mechanistic patient-oriented research to learn translational research techniques to investigate the role of the airway epithelium in pediatric lung diseases and viral infection.

项目总结