CHARACTERIZATION AND ANALYSIS OF PLATELET SETPINS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。septin是一种蛋白质家族，最初在酵母中被鉴定为出芽所必需的。多年来，septin在高等真核细胞中的存在被驳回，因为芽殖，不对称细胞分裂，是酵母特有的过程。然而，最近的研究表明，septin确实存在于高等真核生物中，并且在这些情况下，与涉及膜的动态事件有关。神经生物学实验室最近的工作将SEPT5与神经元的胞外复合体联系起来，暗示SEPT5调节递质释放。在我们的初步研究中，我们证明SEPT5是一个大分子复合物的一部分，该复合物含有与血小板分泌相关的蛋白质syntaxin 4。确定控制血小板分泌的机制是防止血栓形成因子失控释放的关键。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Septins are a family of proteins orginially identified in yeast as essential for budding. For years, the presence of septins in higher eukaryotic cells was dismissed since budding, asymmetric cell division, is a process unique to yeast. However, recent studies have demonstrated that septins do exist in higher eukaryotes and, in those cases examined, as associated with dynamic events involving membranes. Recent work form neurobiology laboratories has associated the septin SEPT5, with exocytic complex of neurons with implications that SEPT5 regulates transmitter release. In our Preliminary Studies, we demonstrate SEPT5 is part of a macromolecular complex containing a protein linked to platelet secretion, syntaxin 4. Defining the mechanisms that control platelet secretion is key to preventing the uncontrolled release of thrombogenic factors.