THE AGE-RELATED INFLUENCES ON INFLAMMATORY MARKERS: A PILOT STUDY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。具体目标：（1）确定年龄与炎症标志物血液水平的关系（细胞因子-CRP、IL-6、TNF;纤溶标志物-纤溶酶原激活物抑制剂-1、组织纤溶酶原激活物和纤维蛋白原），（2）确定身体肥胖、身体活动、身体功能和血管功能是否与炎症标志物的血液水平相关，（3）确定在控制体力活动、身体肥胖和血管功能后年龄的影响是否持续。研究设计和方法：横断面研究设计（N = 100）将允许检查循环血液炎症标志物与年龄之间的关系，而与体力活动水平、功能状态和身体成分无关。为了测量来自血液样品的炎症和纤溶标志物的水平，将采用酶联免疫吸附测定法。此外，将完成通过双能X线吸收测定法进行的身体组成、血管功能（通过小腿血流和动脉弹性）、功能能力和体力活动评估，并在年龄和炎症标志物血液水平之间的研究中进行控制。临床意义：识别与年龄相关的变化，独立于体力活动水平，身体功能，血管功能和身体成分，可以提供相关的见解，反映炎症标志物在心血管疾病的基础病理生理过程中的贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aims: (1) Determine the relationship between age and blood levels of inflammatory markers (Cytokines - CRP, IL-6, TNF; Fibrinolytic markers - plasminogen activator inhibitor-1, tissue plasminogen activator, and fibrinogen), (2) determine if body fatness, physical activity, physical function and vascular function are related to blood levels of inflammatory markers, (3) determine whether the effect of age persists after controlling for physical activity, body fatness, and vascular function. Research Design and Methods: A cross-sectional research design (N = 100) will allow for the examination between circulating blood inflammatory markers and age, independent of physical activity levels, functional status, and body composition. To measure the levels of inflammatory and fibrinolytic markers from a blood sample, an enzyme-linked immunosorbent assay will be employed. Additionally, body composition through dual-energy x-ray absorptiometry, vascular function (via calf blood flow and arterial elasticity), functional capacity and physical activity assessments will be completed and controlled for in the investigation between age and blood levels of inflammatory markers. Clinical Significance: Identifying the age-related changes independent of physical activity levels, physical function, vascular function and body composition may provide pertinent insight reflecting the contribution of the inflammatory markers in underlying pathophysiological processes of cardiovascular disease.