Influences of phytochemicals on age- and disease-related degenerative neurons

植物化学物质对年龄和疾病相关退行性神经元的影响

• 批准号: 21590225
• 负责人: GOTOW Takahiro
• 金额: $ 3.08万
• 依托单位: Koshien University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590225/
• 关键词: レスベラトロール; 老化促進マウス; PC12 細胞; 肝細胞; 活性酸素消去酵素; 細 胞寿命関連タンパク質; ミトコンドリア; オートファジー; PC12細胞; サーチュインタンパク質; AMPK活性化キナーゼ; LC3-I/II タンパク質; LC3遺伝子ノックダウン; 神経細胞; リポフスチン顆粒; 細胞骨格タンパク質; 細胞死関連タンパク質; SIRT蛋白; 細胞死関連蛋白; キサントシン; 細胞骨格系; 細胞死関連蛋白質

We selected red wine polyphenol, resveratrol (RSV), among phytochemicals in this study, and analyzed its effects on neurons and hepatocytes in a neurodegeneration-related senescence-accelerated mouse P10 (SAMP10) and rat neuronal cell line (PC12 cells). When treated with RSV, in the SAMP10 degenerative neurons of the brain were significantly recovered, and as expected in the liver hepatocytes were much more improved. We analyzed more direct influence of RSV on the PC12 cell, and have proposed that RSV may promote cell survival by activations of mitochondria and autophagy.

本研究选择了红酒多酚白藜芦醇(RSV)作为植物化学物质，分析了其对神经变性相关衰老加速小鼠P10(SAMP10)和大鼠神经细胞系(PC12细胞)神经元和肝细胞的影响。经RSV治疗后，SAMP10大鼠脑内变性神经元明显恢复，肝细胞也如预期的那样得到明显改善。我们分析了RSV对PC12细胞更直接的影响，并提出RSV可能通过激活线粒体和自噬来促进细胞存活。

项目成果

Phosphorylation of highly conserved neurofilament medium KSP repeats is not required for myelin-dependent radial axonal growth.

• DOI: 10.1523/jneurosci.3765-08.2009
• 发表时间: 2009-02-04
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Garcia ML;Rao MV;Fujimoto J;Garcia VB;Shah SB;Crum J;Gotow T;Uchiyama Y;Ellisman M;Calcutt NA;Cleveland DW
• 通讯作者: Cleveland DW

Resveratrol is harmful to naive PC12 cells but beneficial to the differentiated ones

白藜芦醇对幼稚 PC12 细胞有害，但对分化细胞有益

• 发表时间: 2009
• 作者: Takeuchi S.;Shiozaki M.;Hayakawa N.,Shibata M.;Koike M.;Uchiyama Y.;Gotow T.
• 通讯作者: Gotow T.

レスベラトロールの細胞生存及び細胞死の調節機構

白藜芦醇对细胞存活和细胞死亡的调节机制

• 作者: Urano T;Suzuki Y;早川直哉
• 通讯作者: 早川直哉

Resveratrol is beneficial to senescence-accelerated mouse neurons and to differentiated but not naive PC12 cells.

白藜芦醇对加速衰老的小鼠神经元以及分化但不幼稚的 PC12 细胞有益。

• 发表时间: 2009
• 作者: Motoko Shiozai;Shoichi Takeuchi;Naoya Hayakawa;Masahiro Shibata;Masato Koike;Yasuo Uchiyama;Takahiro Gotow.
• 通讯作者: Takahiro Gotow.

Neurofilament cross-bridge : A structure associated specifically with the neurofilament among the intermediate filament family

神经丝跨桥：中间丝家族中与神经丝特异性相关的结构

• 期刊: Handbook of Neurochemistry and Molecular Neurobiology 14(in press)
• 作者: Gotow;T
• 通讯作者: T