IDENTIFICATION OF LEUKOCYTE GENES THAT ARE REGULATED BY THYROID HORMONE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。具体目标2包括与国家儿童医学中心的埃里克·霍夫曼博士合作，鉴定其表达水平受甲状腺激素影响的一组白细胞基因。目前正在检测两种特定细胞类型：单核细胞和CD8细胞的基因表达。此外，除了微阵列分析外，我们还计划在参与这项研究的甲减和甲亢受试者中进行检测蛋白质组学。14名甲状腺功能减退症受试者参加并完成了这项研究。一名甲亢受试者参加了这项研究，但不幸的是，他在完成方案之前就失去了后续行动。计划招收20名甲状腺功能减退和20名甲亢患者，共40名。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific aim 2 involves identifying a panel of leukocyte genes whose level of expression is altered by thyroid hormone in collaboration with Dr. Eric Hoffman from Children's National Medical Center. Gene expression in two specific cell types: monocytes and CD8 cells is being examined. In addition, we are also planning to perform detection proteomics, in addition to microarray analyses, in the hypothyroid and hyperthyroid subjects enrolled in this study. 14 hypothyroid subjects have been enrolled in and completed the study. One hyperthyroid subject was enrolled in the subject, but unfortunately was lost to follow-up before he completed the protocol. The planned enrollment is 20 hypothyroid and 20 hyperthyroid subjects for a total of 40 subjects