PROTEOMICS STUDIES OF AIRWAY INFLAMMATION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。简要背景-哮喘是由肺中的信号蛋白调控的。假设-哮喘的信号蛋白可以用新的复杂的实验室技术来识别。特定目的和程序（摘要）-检查过敏原攻击对哮喘和正常受试者肺部蛋白质变化的影响。实验设计（总结）-在吸入刺激后进行支气管镜检查以从肺部获得组织和液体。将分析组织、液体和血液中蛋白质的差异。总体目标是确定哮喘患者中调节过敏原诱导的炎症、粘液分泌和气道高反应性的重要信号蛋白。这些研究将验证ICAT（同位素编码亲和标签）技术在翻译后修饰信号分子的定量蛋白质组学中的有效性。此外，它将开发哮喘气道的信号蛋白质组。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background - Asthma is regulated by signaling proteins in the lung. Hypothesis - The signaling proteins in asthma can be identified with new sophisticated laboratory techniques. Specific Aims and Procedures (summary) - To examine the effects of allergen challenge on the changes in proteins present in the lungs for asthmatic and normal subjects. Experimental Design (summary) - Perform bronchoscopy to obtain tissue and fluid from the lung after an inhalation challenge. Tissue, fluid, and blood will be analyzed for differences in proteins. The overall goal is to identify important signaling proteins that regulate allergen-induced inflammation, mucus secretion and airway hyperreactivity in asthmatic patients. These studies will validate the usefulness of ICAT (isotope-coded affinity tag) technology for quantitative proteomics of posttranslationally modified signaling molecules. Further it will develop a signaling proteome for asthmatic airways.