CORE: SIGNAL TRANSDUCTION CORE FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。受体-配体相互作用和信号转导生物化学的诱导是细胞生物学中所有调节事件的驱动力。这种调节在免疫系统中尤其明显，它表现出许多受体-配体相互作用，促进免疫细胞分化、增殖、凋亡和效应功能的激活。因此，信号转导生物化学是理解免疫细胞内稳态生物事件的核心。在过去的十年中，免疫细胞信号传导的知识体系已经相当成熟，特别是随着酪氨酸蛋白激酶在调节蛋白-蛋白相互作用中的中心作用的发现。由于这是一个新兴领域，用于研究细胞因子或其他免疫细胞激活剂诱导的信号事件的技术相当专业化，没有直接支持的经验不足的实验室可能不易获得。考虑到从一个受体到另一个受体的分析的相似性，我们期望从过去的经验中受益于那些研究相关信号系统的人。因此，本申请建议继续支持一个核心设施，以容纳专注于免疫细胞信号转导生物化学的专业知识和设备。它将被用作需要探索各种免疫反应中的信号事件的新研究人员的知识库和技术资源，并将促进专业知识和特定分析的开发和转让给研究其他信号网络的研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Receptor-ligand interactions and the induction of signal transduction biochemistry are the driving force for all regulatory events in cell biology. This regulation is especially evident in the immune system, which exhibits numerous receptor-ligand interactions that promote immune cell differentiation, proliferation, apoptosis, and activation of effector functions. Accordingly, signal transduction biochemistry is central to understanding the biological events within immune cell homeostasis. The body of knowledge of immune cell signaling has matured considerably over the past ten years, especially with the discovery of the central role for tyrosine protein kinases in regulatory protein-protein interactions. Since it is a nascent field, the techniques used to study signaling events induced by cytokines or other immune cell activators are fairly specialized and perhaps not readily acquired by inexperienced laboratories without direct support. Given the similarity of assays that apply from one receptor to another, we expect benefit from past experience for those who study related signaling systems. Thus, this application proposes to continue to support a core facility to house expertise and equipment that focuses on immune cell signal transduction biochemistry. It will be used as a knowledge-base and technical resource for new investigators who find a need to explore signaling events in various immune responses, and will facilitate development and transfer of expertise and specific assays to researchers studying other signaling networks.