COBRE: UID: MOLECULAR BIOLOGY CORE FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。分子生物学核心设施为研究人员提供了最先进的仪器，设备和设施，用于分析核酸序列。核心设施现占地850平方米。该实验室位于生命科学南部，新装修的空间有20英尺，并配备了下文所述的主要仪器和计算资源，以及湿实验室工作台、两个NuAire生物安全柜、热循环仪、离心机、微量离心器、冷冻机、冰箱和样品制备所需的其他次要设备。一名全职技术人员监督仪器的操作和维护，并为设施的用户提供说明、指导和故障排除帮助。核心设施现有的主要仪器包括：两台毛细管DNA测序仪（Applied Biosystems PRISM 3730 DNA分析仪和ABI 3730是48毛细管系统），一台Qiagen BioRobot 3000，用于使用96孔微孔板格式进行高通量样品处理。实验室的一部分专门用于数据分析和生物信息学。该实验室配备了两台Macintosh Power Mac G5计算机，具有双2 GHz处理器，1GB RAM和两个250 GB硬盘驱动器，通过安全的高速本地网络进行联网。这为研究人员提供了在互联网上和通过生物信息学核心设施获得资源的机会。这些是最先进的设施，支持爱达荷州大学的广泛生物医学研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core Facility has provided researchers access to state-of-the-art instruments, equipment, and facilities for the analysis of nucleic acid sequences. The Core Facility now occupies 850 sq. feet of newly renovated space in Life Sciences South, and is equipped with the major instrumentation and computational resources described below, as well as wet lab benches, two NuAire biosafety cabinets, thermocyclers, centrifuges, microfuges, freezers, refrigerators, and other minor equipment needed for sample preparation. A full time technician oversees operation and maintenance of the instruments, and provides instruction, guidance, and trouble-shooting assistance to users of the facility. The major instrumentation now available in the Core Facility includes the following: two capillary DNA sequencers (an Applied Biosystems PRISM 3730 DNA Analyzer, and an ABI 3730 is a 48 capillary system), a Qiagen BioRobot 3000 for high thoroughput sample processing using a 96-well microplate format. A portion of the laboratory is dedicated to data analysis and bioinformatics. The laboratory is equipped two Macintosh Power Mac G5 computers which have dual 2 GHz processors, 1GB of RAM, and two 250 GB hard drives that are networked through a secure high-speed local network. This provides investigators with access to resources that are available on the Internet and through the Bioinformatics Core Facility. These are state of the art facilities that support a wide range of biomedical research projects at the University of Idaho.