PROTEOMICS FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。PRAABRE项目的蛋白质组学设施位于波多黎各大学里约热内卢校区(UPR-RP)。该设施的建立是为了使PRAABRE项目的研究人员以及UPR-RP和邻近机构的其他研究人员能够更好地了解与生物医学研究相关的重要细胞和分子过程。特别是，该设施提供了必要的技术和专业知识，以协助科学界进行特定的分子生物学实验。这一核心设施为分享成像分析、分子克隆、Southern、Northern和Western blotting、单和双荧光素酶分析以及蛋白质表达和纯化等技术方面的专业知识和培训提供了机会。蛋白质组学设施的工作人员能够在不同领域培训和帮助研究人员和学生，包括：核酸提纯、DNA和RNA的酶操作、聚合酶链式反应、克隆、定点突变、重组DNA分子的构建、重组蛋白质的表达和纯化。蛋白质组学基金的活动侧重于咨询、教学和合作。咨询活动的范围从简单的实验建议和故障排除，到帮助实验设计和将想法和初步数据纳入提案。此外，该设施可以为希望在开发与重组DNA、蛋白质表达和纯化相关的新项目方面提供帮助的调查人员提供技术支持和试剂，这些项目他们可能不熟悉。核心设施的实验室人员、研究生和本科生也可以进行结构化和个性化教学。这一核心设施的未来发展包括分析性双向凝胶电泳和必要的工具，以对细胞蛋白质组进行高通量分析，描述不同细胞状态下的蛋白质变化，并确定纯化蛋白质的结构和化学性质。这一最先进的设施将配备超高灵敏度质谱仪以及蛋白质分离和电泳系统。总之，这些活动对于提高PR-AABRE项目和蛋白质组学基金的研究人员的科学和教育能力是必要的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Proteomics Facility of the PRAABRE Project is located at the University of Puerto Rico-Rio Piedras Campus (UPR-RP). This facility has been created to enable the investigators of the PRAABRE Project as well as other researchers at UPR-RP and at neighboring institutions to better understand important cellular and molecular processes that are relevant to biomedical research. In particular, this facility provides the necessary technology and expertise to assist the scientific community in conducting specific molecular biology experiments. This core facility provides an opportunity for sharing expertise and training in such techniques as imaging analysis, molecular cloning, Southern, Northern and Western blotting, single and dual luciferase assays and protein expression and purification. The personnel at the Proteomics Facility are capable of training and helping investigators and students in different areas including: nucleic acid purification, enzymatic manipulation of DNA and RNA, PCR, cloning, site-directed mutagenesis, and construction of recombinant DNA molecules, expression and purification of recombinant proteins. The activities of the Proteomics Facility are focused on consulting, teaching, and collaboration. Consulting activities range from simple experimental advice and troubleshooting to assistance in experimental design and incorporation of ideas and preliminary data into proposals. In addition, the facility can provide technological support and reagents to investigators wishing assistance in developing new projects related to recombinant DNA, protein expression and purification with which they may not be familiar. Both structured and individualized teaching is also available to laboratory personnel, graduate and undergraduate students at the core facility. Future developments of this core facility include analytical two-dimensional gel electrophoresis and the necessary tools to conduct high-throughput analyses on cellular proteomes, profile protein changes under different cellular states and determine the structural and chemical properties of purified proteins. This state-of-the-art facility will be equipped with ultra-high sensitive mass spectrometer and protein separation and electrophoresis systems. All together, these activities are necessary to enhance the scientific and educational capabilities of both the researchers of the PR-AABRE project and the Proteomics Facility.