Ion mobility mass spectrometer for proteomics facility

用于蛋白质组学设施的离子淌度质谱仪

• 批准号: BB/T01783X/1
• 负责人: Alex Jones
• 金额: $ 56.51万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT01783X%2F1
• 关键词: Ion; mobility; mass; spectrometer; proteomics

Mass spectrometry is a powerful analytical method that is used to identify and quantify compounds from a huge variety of materials, for example, identify enzymes engaged with degradation of waste in soil, or to understand how a cell can divide into two. Mass spectrometry can be used on any type of molecule that can carry a positive or negative charge and is stable in the gas phase at high vacuum. In the Proteomics Research Technology Platform at the University of Warwick we use mass spectrometry to study proteins, which we usually break into small pieces called peptides to make high throughput, large-scale analysis feasible. Proteins provide many vital functions in our bodies from making structures like collagen to working as enzymes to digest our food. In this proposal, we request a new type of mass spectrometer that can separate charged molecules (ions) by their 'shape' (really cross-collisional dimension) as well as separating ions by mass and charge, as all mass spectrometers do. This extra ability, called ion-mobility, would provide us with a very flexible and exciting instrument, ideal for measuring and quantifying peptides from liquid solutions. Ion mobility means that we would be able to distinguish between very similar peptides that have the same mass, but have, for example, a different order of amino acids, like PEPTIDE and PPEETID. This would be a great improvement over our current instrumentation, where we sometimes see evidence that we have accidently fragmented two different peptides at the same time, which makes the result (the scan) more difficult to interpret. If funded, we would prioritise time for researchers in BBSRC science areas, such as meeting challenges in Food Security, by deciphering how protein signalling helps plants defend themselves against pathogens. Or by understanding fundamental challenges in how life works, for example in discovering the molecular details of how a yeast cell divides into two using a contractile ring. We have already developed innovative tools to help teach students and explain mass spectrometry to the public. For example, our animation and "ping-pong ball mass spectrometer" are very popular at open days and can be found on youtube. If funded, we would create new tools to demonstrate ion mobility and invite you to visit us on one of our open days in 2021.

质谱法是一种强大的分析方法，用于识别和量化各种材料中的化合物，例如，识别参与土壤中废物降解的酶，或了解细胞如何分裂成两个。质谱法可以用于任何类型的分子，可以携带正电荷或负电荷，并在高真空下的气相中稳定。在沃里克大学的蛋白质组学研究技术平台中，我们使用质谱法来研究蛋白质，我们通常将其分解成称为肽的小片段，以使高通量，大规模分析成为可能。蛋白质在我们的身体中提供许多重要功能，从制造胶原蛋白等结构到作为酶消化我们的食物。在这个提议中，我们要求一种新型的质谱仪，可以通过它们的“形状”（真正的交叉碰撞维度）分离带电分子（离子），以及通过质量和电荷分离离子，就像所有的质谱仪一样。这种额外的能力，称为离子迁移率，将为我们提供一种非常灵活和令人兴奋的仪器，非常适合测量和定量液体溶液中的肽。离子迁移率意味着我们将能够区分具有相同质量但具有不同氨基酸顺序的非常相似的肽，如肽和PPEETID。这将是对我们目前仪器的巨大改进，我们有时会看到我们同时意外地将两种不同的肽片段化的证据，这使得结果（扫描）更难以解释。如果获得资助，我们将优先考虑BBSRC科学领域的研究人员的时间，例如通过破译蛋白质信号如何帮助植物防御病原体来应对粮食安全的挑战。或者通过了解生命如何运作的基本挑战，例如发现酵母细胞如何使用收缩环一分为二的分子细节。我们已经开发了创新的工具来帮助学生教学和向公众解释质谱。例如，我们的动画和“乒乓球质谱仪”在开放日非常受欢迎，可以在youtube上找到。如果获得资助，我们将创建新的工具来展示离子迁移率，并邀请您在2021年的开放日参观我们。

项目成果

Cdk1-mediated threonine phosphorylation of Sam68 modulates its RNA binding, alternative splicing activity and cellular functions.

• DOI: 10.1093/nar/gkac1181
• 发表时间: 2022-12-09
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Malki, Idir;Liepina, Inara;Kogelnik, Nora;Watmuff, Hollie;Robinson, Sue;Lightfoot, Adam;Gonchar, Oksana;Bottrill, Andrew;Fry, Andrew M.;Dominguez, Cyril
• 通讯作者: Dominguez, Cyril

A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3.

• DOI: 10.7554/elife.81755
• 发表时间: 2022-09-06
• 期刊: eLife
• 影响因子: 7.7
• 作者: Pardal AJ;Bowman AJ
• 通讯作者: Bowman AJ