SCIENCE AND RESEARCH CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。科学研究核心提供功能基因组学的仪器和专业知识，以支持缅因州INBRE成员机构的研究项目。核心支持细胞和分子生物学、电生理学和高分辨率成像方面的活动。在本报告所述期间，核心提供了细胞培养、流式细胞术和细胞系开发、共聚焦显微镜、DNA测序、RNA纯化和分析以及通过定量聚合酶链式反应进行基因表达分析的服务。海洋模式物种归一化混合组织cDNA文库的表达序列标签的GenBank提交正在进行中。为比较基因组模型开发的新细胞培养包括鲨鱼、溜冰的成年组织或胚胎的细胞培养，以及几种河豚细胞系也从斑马鱼、贝壳鱼和其他淡水鱼模式物种发展而来。在过去的一年里，该单位开发了流式细胞术的新程序，并将其应用于比较基因组学模型。该中心通过建立基因导入缅因州INBRE项目中使用的一些比较模型的转基因技术，进一步加强了生物医学研究。科学研究中心还提供细胞生物学和功能基因组学方法学方面的培训和个人咨询，并出版了一套共同的分子生物学方案。此外，中心还通过提供本科生和研究生指导、博士后培训以及仪器和服务来支持缅因州INBRE的教学计划。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Science Research Core provides instrumentation and expertise in functional genomics in support of research projects at Maine INBRE member institutions. The Core supports activities in cell and molecular biology, electrophysiology, and high resolution imaging. During the reporting period, the Core provided services in cell culture, flow cytometry and cell line development, confocal microscopy, DNA sequencing, RNA purification and analysis, and gene expression analysis via quantitative PCR. GenBank submission of expressed sequence tags for normalized mixed tissue cDNA libraries of marine model species is in progress. Novel cell cultures developed for comparative genomic models include those from adult tissues or embryos of shark, skate and several species of pufferfish Cell lines also have been developed from zebrafish, platyfish and other freshwater fish model species In the past year the unit has developed and applied new procedures for flow cytometry to models of comparative genomics. The Core has further enhanced biomedical research by establishing transfection technology for introduction of genes into a number of the comparative models used in the Maine INBRE project. The Science Research Core also provided training and individual consultation in cell biology and functional genomics methodology and publishes a set of common molecular biological protocols. In addition, the Core supports the teaching program of Maine INBRE by providing undergraduate and graduate student instruction and postdoctoral training as well as instrumentation and services.