Thrips Resistance In Tomato Plants

代谢组学已经迅速发展成为一项有价值的技术，在弥合表型和基因型之间的知识差距方面发挥着不可或缺的作用。与生态学方法一起，它将遗传学与健康和功能联系起来。尽管人们普遍认为，一个成功的应用基因组学项目需要分子、遗传、代谢组学和生态学方法的结合，但我们的项目是第一个整合这些专业知识的项目之一。我们打算研究一种酚类化合物，绿原酸（CGA），它在植物中作为抗氧化剂，有望防止退行性疾病，与年龄有关的疾病。我们的项目将研究CGA对植物抗草食性昆虫的重要性。具体来说，我们希望：-利用第二代分子方法，通过增加天然CGA水平，开发出对西方花蓟马（Frankliniella occidentalis）具有抗性的番茄品系。-探索番茄中CGA水平的自然变化-增加我们对CGA在蓟马抗性中的作用的理解-确定CGA产生水平背后的遗传学-了解CGA与初级和次级代谢之间的关系-为后基因组研究建立持久的研究网络

Metabolomics has rapidly developed into a valuable technology playing an indispensable role in bridging the gaps in knowledge between phenotypes and genotypes. Together with ecological approaches it links genetics to fitness and function. Despite the general belief that a succesfull applied genomics project requires a combination of molecular, genetic, metabolomic and ecological approaches our programme is one of the first to integrate this range of expertise. We intend to study a phenolic compound, chlorogenic acid (CGA), which acts as an antioxidant in plants and is expected to protect against degenerative, age related diseases. Our programme will study the importance of CGA for plant resistance against herbivorous insects. Specifically we want: - to develop tomato lines resistant to western flower thrips (Frankliniella occidentalis) by increasing natural CGA levels, using a second generation molecular approach. - to explore the natural variation in CGA levels in tomatoes - to increase our understanding of the role of CGA in thrips resistance - to identify the genetics behind CGA production levels - to understand the relationships between CGA and the primary and secondary metabolism - to establish a long-lasting research network for post-genomic research