14 ERA-CAPS Regulating Tomato quality through Expression (RegulaTomE)

14 ERA-CAPS 通过表达调节番茄质量 (RegulaTomE)

• 批准号: BB/N005023/1
• 负责人: Cathie Martin
• 金额: $ 78.9万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN005023%2F1
• 关键词: 14; ERA; CAPS; Regulating; Tomato

The twin objectives of RegulaTomE are to determine the importance of transcriptional regulation of the metabolic pathways defining quality traits in tomato and to identify such transcriptional regulators at the molecular level. The selected quality traits include those determining antioxidant capacity which impacts shelf life and nutritional value as well as those determining fruit flavor and over-ripening which influence organoleptic properties and shelf-life. Loci contributing to abiotic stress tolerance will also be identified toward the combined goals of more nutritious, stable and sustainable crops. RegulaTomE will use the natural variation available in introgression lines (ILs) resulting from wild species crosses to tomato to assess the importance of transcriptional regulation, identify additional regulatory genes and assess underlying genetic and epigenetic variation. RegulaTomE will additionally assess the potential for direct or indirect use of natural variation from a largely untapped wild species resource for crop improvement. To identify genes regulating metabolic pathways using the Solanum lycopersicoides ILs, and to capture as much genetic and epigenetic variation as possible for application to gene discovery and tomato improvement, resources need to be developed, including a genome reference sequence for S. lycopersicoides and metabolite, DNA methylation and transcriptome profiles of IL fruit. Resulting data will be made available without restriction via existing public databases, providing invaluable resources for the community to exploit further the untapped natural variation in S.lycopersicoides. RegulaTomE will lead to regulatory gene identification (an important advance in terms of fundamental understanding), and provide new tools for metabolic engineering of fruit quality. More immediately, the natural variation in fruit quality revealed by the S.lycopersicoides ILs could be applied to tomato improvement either directly through introgression into cultivated varieties or indirectly through the identification of target loci and corresponding allelic variation making positive contributions to quality traits within S. lycopersicum breeding germplasm and in other close relatives of tomato. The cooperation of scientists from five major European organisations and two US Universities producing world-leading research on fleshy fruit and agricultural/horticultural innovation will allow the development of tools and resources on a scale unavailable at a national level. The outputs of RegulaTomE will provide a framework of understanding as well as tools, in the form of genes, target loci and molecular markers, to support development of longer shelf-life, more nutritious and more flavorsome fleshy fruits in other horticultural crops. The effectiveness of these resources and the significance of the knowledge acquired on RegulaTomE will ensure that the project contributes directly to food security and sustainable fruit cultivation.

RegulaTomE的双重目标是确定转录调控的重要性，在番茄中定义质量性状的代谢途径，并确定这样的转录调节因子在分子水平上。所选的质量性状包括那些决定影响货架期和营养价值的抗氧化能力的性状，以及那些决定影响感官特性和货架期的水果风味和过熟的性状。还将确定有助于非生物胁迫耐受性的基因座，以实现更有营养、稳定和可持续的作物的综合目标。RegulaTomE将利用野生种与番茄杂交产生的渐渗系（IL）中的自然变异来评估转录调控的重要性，鉴定其他调控基因，并评估潜在的遗传和表观遗传变异。RegulaTomE还将评估直接或间接利用大部分未开发的野生物种资源的自然变异进行作物改良的潜力。为了利用类番茄茄IL鉴定调节代谢途径的基因，并捕获尽可能多的遗传和表观遗传变异以应用于基因发现和番茄改良，需要开发资源，包括S. IL果实的类番茄和代谢物、DNA甲基化和转录组谱。由此产生的数据将不受限制地通过现有的公共数据库，为社会提供宝贵的资源，进一步利用S. lycopersicoides的未开发的自然变异。RegulaTomE将导致调控基因的鉴定（在基本理解方面的重要进展），并为水果品质的代谢工程提供新的工具。更直接的是，由类番茄自交系揭示的果实品质的自然变异可以直接通过渐渗到栽培品种中来应用于番茄改良，或者间接地通过鉴定目标基因座和相应的等位基因变异来应用于番茄改良，所述等位基因变异对类番茄自交系内的品质性状做出积极贡献。番茄育种种质和番茄的其他近缘种。来自五个主要欧洲组织和两所美国大学的科学家在肉质水果和农业/园艺创新方面进行了世界领先的研究，他们的合作将使工具和资源的开发在国家层面上无法实现。RegulaTomE的成果将以基因、靶位点和分子标记的形式提供一个理解框架和工具，以支持在其他园艺作物中开发更长货架期、更有营养和更美味的肉质水果。这些资源的有效性和RegulaTomE获得的知识的重要性将确保该项目直接促进粮食安全和可持续水果种植。

项目成果

Voices of biotech.

生物技术的声音。

• DOI: 10.1038/nbt.3502
• 发表时间: 2016
• 期刊: Nature biotechnology
• 影响因子: 46.9
• 作者: Amit I

Diverting tyrosine: Data from untargeted metabolic analysis of tomato fruit accumulating L-DOPA.

• DOI: 10.1016/j.dib.2020.106678
• 发表时间: 2021-03
• 期刊: Data in brief
• 影响因子: 1.2
• 作者: Breitel D;Brett P;Alseekh S;Fernie AR;Butelli E;Martin C
• 通讯作者: Martin C

Beyond Purple Tomatoes: Combined Strategies Targeting Anthocyanins to Generate Crimson, Magenta, and Indigo Fruit

• DOI: 10.3390/horticulturae7090327
• 发表时间: 2021-09-01
• 期刊: HORTICULTURAE
• 影响因子: 3.1
• 作者: Butelli, Eugenio;Bulling, Katharina;Martin, Cathie
• 通讯作者: Martin, Cathie

Identification of transcriptional regulators determining nutritional quality of tomato

确定番茄营养品质的转录调节因子的鉴定

• 发表时间: 2019
• 作者: Fearnley Eleanor

Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato

• DOI: 10.1007/s11434-016-1108-9
• 发表时间: 2016-09-01
• 期刊: SCIENCE BULLETIN
• 影响因子: 18.9
• 作者: Ewas, Mohamed;Gao, Yanqiang;Luo, Jie
• 通讯作者: Luo, Jie