Impact of elevated temperatures and limiting water supply on virus resistance of tomato plants

气温升高和限制供水对番茄植株病毒抗性的影响

• 批准号: 208480791
• 负责人: Professor Dr. Uwe Sonnewald
• 金额: --
• 依托单位: Lehrstuhl für Biochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/208480791?language=en
• 关键词: Impact; elevated; temperatures; limiting; water

Climate changes are likely to impact plant diseases. Especially drought and heat stress will influence plant-virus interactions. Here we propose to study mechanisms of resistance to Tomato spotted wilt virus (TSWV) and Potato virus Y (PVY) of tomato plants exposed to heat and drought stress. Both viruses belong to different virus families with devastating impact on tomato yield worldwide. In addition, Tsw-mediated TSWV resistance is unstable under elevated temperatures, which makes development of novel resistance strategies a necessity. To achieve these goals, we propose to generate transgenic tomato plants with altered expression of TSWV or PVY interacting host proteins and to validate resistance of these transgenic plants under heat and drought stress. To study the possible suppression of virus resistance by heat and drought stress, transgenic plants and additional tomato genotypes carrying resistance genes conferring potyvirus and tospovirus resistance will be challenged with elevated temperatures and limiting water supply prior infection with different PVY and TSWV isolates under controlled and field conditions. Monitoring virus replication as well as metabolic and transcriptional changes will allow a comparative analysis linking specific transcript and metabolite changes to susceptible and resistant host-virus combinations and will help to design durable heat- and drought-stable virus resistance in tomato plants.

气候变化可能会影响植物病害。特别是干旱和高温胁迫会影响植物与病毒的相互作用。本文旨在研究高温干旱胁迫下番茄对番茄斑萎病毒（TSWV）和马铃薯Y病毒（PVY）的抗性机制。这两种病毒属于不同的病毒家族，对全球番茄产量具有破坏性影响。此外，TSW介导的TSWV抗性在升高的温度下是不稳定的，这使得开发新的抗性策略成为必要。为了实现这些目标，我们建议产生TSWV或PVY相互作用宿主蛋白表达改变的转基因番茄植物，并验证这些转基因植物在高温和干旱胁迫下的抗性。为了研究热和干旱胁迫对病毒抗性的可能抑制，将在受控和田间条件下用不同的PVY和TSWV分离物感染之前，用升高的温度和限制供水来挑战转基因植物和携带赋予马铃薯Y病毒属和番茄斑萎病毒属抗性的抗性基因的另外的番茄基因型。监测病毒复制以及代谢和转录变化将允许比较分析将特定转录物和代谢物变化与易感和抗性宿主病毒组合联系起来，并将有助于设计番茄植物中持久的耐热和耐旱病毒抗性。