CELL TO CELL COMMUNICATION IN MYCOBACTERIA (NEW PROJECT TITLE)

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在张博士的实验室里，我继续研究eb病毒和癌症之间的关系。eb病毒（EBV）是一种人类疱疹病毒，在哺乳动物细胞的肿瘤发生中起着关键作用。转化过程主要由EBV潜伏膜蛋白1 （LMP-1）引起。在体外，如果没有LMP-1的表达，EBV不能转化原代b细胞。已经产生了LMP-1显性负突变体，称为LMP-DM。LMP-DM蛋白具有抑制通常由LMP-1调节的细胞信号分子的能力，特别是那些参与癌症形成的分子。本研究的目的是测试LMP-DM是否具有阻断EBV转化细胞中LMP-1转化功能的能力。第二个项目（2004年5月- 2004年12月）是我研究的人类疱疹病毒8 （HHV-8）、eb病毒（EBV）和体腔肿瘤形成之间的关系。利用含有HHV-8和EBV DNA的载体进行共转染实验，并进行免疫沉淀实验，以确定HHV-8和EBV是否导致肿瘤形成，特别是通过在体内引起肿瘤特异性蛋白的表达。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In Dr. Zhang's lab I have continued researching the relationship between the Epstein Barr Virus and cancer. Epstein-Barr virus (EBV), a human herpesvirus, plays a critical role in oncogenesis of mammalian cells. The transformation process is primarily caused by the EBV latent membrane protein 1 (LMP-1). In vitro, EBV cannot transform primary B-cells without the expression of LMP-1. A dominant negative mutant of LMP-1 referred to as LMP-DM has been generated. The LMP-DM protein has the ability to inhibit cell signaling molecules which are normally regulated by LMP-1, specifically those involved in cancer formation . The purpose of this research was to test if LMP-DM has the ability to block the transformation function of LMP-1 in EBV transformed cells. A second project (May 2004-Dec2004) that I have worked on is looking at the relationship between the Human Herpesvirus 8 (HHV-8), Epstein Barr Virus (EBV), and body cavity tumor formation. Co-transfection experiments using vectors containing DNA from HHV-8 and EBV along with immunoprecipitation experiments were done to determine if HHV-8 and EBV cause tumor formation, specifically by causing the expression of tumor specific proteins in vivo.