CONSERVATION GENETICS OF THE AMERICAN DIPPER IN THE BLACK HILLS OF SOUTH DAKOTA

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这个项目的长期目标是为美洲美洲斗蟋（Cinclus mexicanus）开发DNA指纹标记，这可以用来确定布莱克山种群与落基山脉其他种群的关系。1996年，美国北斗七星被列入南达科他州的濒危物种名单。这种小型水鸟的数量在整个南达科他州都在下降？除了斯皮尔菲什溪以外，其他的河流都有。潜在筑巢地点的丧失和溪流的筑坝导致了北斗七星数量的下降（Price and Bock, 1983）。此外，河流淤积的增加对水生昆虫幼虫有害，而水生昆虫幼虫是美洲鲈鱼的主要食物来源。该项目始于2002年1月，开发了一种非侵入性的方法，从布莱克山的小白鲸种群中获取DNA样本。这很重要，因为需要在不进一步减少种群规模的情况下收集遗传数据。该项目的下一阶段是构建小片段基因组文库，并在北斗卫星基因组中识别多态微卫星区域。然后，我们将利用这些区域开发遗传标记。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term objective of this project is to develop DNA fingerprinting markers for Cinclus mexicanus, the American dipper, that can be used to determine the relationship of the Black Hills populations with other Rocky Mountain populations of this species. In 1996 the American dipper was placed on the list of threatened species in South Dakota. The population of this small aquatic bird is in decline in all of South Dakota?s streams except for Spearfish Creek. Loss of potential nesting sites and damming of streams have contributed to the declining dipper populations (Price and Bock, 1983). Additionally, the increasing sedimentation of streams has been detrimental to aquatic insect larvae, the major food source of American dippers. The project, which began in January 2002, has developed noninvasive methods to obtain DNA samples from the Black Hills population of dippers. This is significant because of the need to gather genetic data without further reducing population size. The next phase of the project is to construct a small fragment genomic library and identify polymorphic microsatellite regions within the dipper genome. We would then develop genetic markers using those regions.