COBRE: USC: MOUSE CORE, COLON CANCER

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。小鼠研究核心设施继续为结肠癌研究中心（CCCR）的研究人员提供动物研究支持。 Mouse Core支付目标研究者使用的所有小鼠群体的饲养笼费用，提供用于小鼠PCR基因分型的所有设备和试剂，以及各种研究的组织采购，以及动物程序的技术支持和专业知识。 在过去的一年里，我们增加了以下设备和能力：BTX 830 ElectroSquare电穿孔系统，与异氟烷小动物麻醉机一起，为小动物手术和体内肌内电穿孔提供了一个完全集成的系统; Baker SG 303 SterilGARD III层流罩和NAPCO组织培养箱，以满足COBRE研究人员的需求。 我们与CCCR病理学中心合作，从ApcMin/+小鼠和Per 2 ApcMin/+小鼠中收集组织，对这些小鼠的组织进行病理学调查。这一合作成果发表在《国际实验病理学杂志》上。我们还与组织学和成像中心合作，检查ApcMin/+的小肠和结肠中造血干细胞（HSC）的植入，并检查移植耐药HSC对胸苷酸合成酶抑制剂的肿瘤反应的影响。一份手稿正在准备提交给《癌症研究》杂志。几位新的研究者，包括洛恩·霍夫塞斯（药理学）、丹·狄克逊（生物学和南卡罗来纳州癌症中心）和特洛伊·鲍迪诺（医学院），增加了他们对小鼠的使用。Mouse Core为这些研究人员提供笼子费用，并协助他们培训人员和采购供其使用的小鼠品系。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Mouse Research Core Facility continues to provide animal research support to the investigators of the Center for Colon Cancer Research (CCCR). The Mouse Core pays for all the cage charges incurred in maintaining all mouse colonies utilized by the target investigators, provide all equipment and reagents for PCR genotyping of mice and procurement of tissues for various studies, as well as technical support and expertise in animal procedures. In the past year, we have added the following equipment and capabilities: a BTX 830 ElectroSquare Electroporation System, which together with the isoflurane small animal anesthesia machine, provides a fully integrated system for small animal surgeries and in vivo intramuscular electroporation; and a Baker SG303 SterilGARD III laminar flow hood and a NAPCO tissue culture incubator to meet the needs of COBRE inestigators. We collaborated with the CCCR Pathology Core to collect tissues from the ApcMin/+ mouse and the Per2 ApcMin/+ mouse to carry out a pathological survey on tissues from these mice. This collaboration has resulted in a publication in the International Journal of Experimental Pathology. We also collaborated with the Histology and Imaging Core to examine the engraftment of hematopoietic stem cells (HSCs) in the small intestine and colon of the ApcMin/+ and to examine the effects of transplantation of drug resistant HSCs on tumor response to thymidylate synthase inhibitors. A manuscript is in preparation for submission to the journal Cancer Research. Several new investigators, including Lorne Hofseth (Pharmacology), Dan Dixon (Biology and South Carolina Cancer Center), and Troy Baudino (School of Medicine), have increased their use of mice. The Mouse Core provided the cage costs for these investigators, in addition to assisting them in training their personnel and procuring mouse strains for their use.