COBRE: RW HOSP: NEW STEM CELL BIOLOGY: CELL SORTER/FLOW CYTOMETRY CORE FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。细胞分选仪核心将提供骨髓或皮肤干细胞分离和细胞悬液中供体细胞分析的专业知识。该核心将由Nicola Kouttab博士领导。Koutab博士在RWMC经营流动设施，并拥有经验丰富的技术人员。此外，Mark Dooner先生是一位经验丰富的干细胞/分选技术人员，将投入一定比例的时间在这一核心领域。与Koutab博士一起工作的其他技术人员将能够提供后备和交叉覆盖，尽管他们不是核心的正式组成部分。该核心采用Cytomation的MoFIo高速细胞分选仪。该分选机能够进行干细胞分离、单细胞沉积和大事件分析。该核心容纳在已建立的流式细胞术设施内的专用房间中。该FACS设施配备有FACS扫描仪和FACS calibur（BD仪器）。这两台机器都可以作为项目所需的任何分析工作的备份。研究人员将与核心工作，并准备他们的细胞群进行分类或分析。核心将常规分离Lin-Rho（lo）Ho（lo）、Lin-Hoechst侧群和Lin-Sca + CD 45-细胞，并分析GFP+或CFDA-SE阳性细胞群。核心将服务于COBRE中的所有项目，为项目1、2、3进行分离和分析工作。核心也仍然致力于开发新的和增强已知的干细胞分离。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core will provide expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. The core will be headed by Dr. Nicola Kouttab. Dr. Koutab runs the flow facility at RWMC and has experienced technicians. In addition, Mr. Mark Dooner is an experienced stem cell/sorter technician and will devote a percentage of his time to this core. The other technicians working with Dr. Koutab will be able to provide backup and cross coverage although they are not a formal component of the Core. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. This core is housed in a dedicated room within an established flow cytometry facility. This FACS facility is equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators will work with the core and prepare their cell populations for sorting or analysis. The core will routinely separate Lin- Rho(lo)Ho(lo),Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core will serve all projects in this COBRE carrying out both separations and analysis work for projects 1, 2, 3. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.