Application of MesoScale Technology for the Measurement of Multiple Biomarkers to Explore Molecular and Cellular Processess.

分子和细胞事件的分析在现代生命科学研究中至关重要，可以更好地理解（一）基本生物过程，（二）表征相关疾病状态的变化，以及（三）治疗方法的发展。该研究计划汇集了七个研究领域，涵盖免疫和神经系统的调节，造血，组织发育和再生以及细胞代谢，这些领域都有一个共同的主题，即希望利用敏感，定量和高通量的最先进仪器来分析一系列细胞机制。该仪器是中尺度发现（MSD）扇区成像仪（SI） 6000，它提供了一个敏感的，适应性强的平台，用于测量细胞或细胞衍生样品中的几种分析物。该技术依赖于使用化学标记抗体来识别所选择的分析物。当与目标结合时，Abs在电化学刺激下发光，这是测量和量化的。该仪器提供的主要特点是：-能够准确量化特定信号的激活；-方便同时检测多个信号。-在细胞样本有限的情况下，可以同时生成几种不同信号分子的信息。-该系统允许更快速的分析，-为开发我们自己的定制分析提供平台，用于未来的应用。

Analysis of molecular and cellular events is crucial in modern life sciences research, for a better understanding of (i) fundamental biological processes, (ii) the changes that characterise associated disease states, and (iii) the development of therapeutics. This research proposal brings together seven research areas, spanning the regulation of the immune and nervous systems, haemopoiesis, tissue development and regeneration and cell metabolism that share a common theme of wanting to utilise sensitive, quantitative and high throughput state-of-the-art instrumentation to analyse a range of cellular mechanisms. This instrument is the Mesoscale Discovery (MSD) SECTOR Imager (SI) 6000 which offers a sensitive, adaptable platform with which to measure several analytes in cell or cell-derived samples. The technology relies on the use of chemically tagged antibodies that recognize the analytes of choice. When bound to their target, the Abs emit light upon electrochemical stimulation which is measured and quantified. The main features provided by the instrument are: - the ability to accurately quantify activation of specific signals; - it facilitates detection of multiple signals simultaneously. - in situations where cell samples may be limiting, information on several different signalling molecules can be generated simultaneously. - the system allows for more rapid analysis, - provides platform for developing our own custom made assays for future applications.