DIETARY PROTEIN COMPOSITION AFFECTS PROTEIN KINETIC RESPONSE IN MARASMIC CHILD

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。本研究比较了亮氨酸动力学和急性时相蛋白浓度在三组消瘦，急性感染马拉维儿童喂养的三个等能饮食之一。这些问题是：提高蛋白质质量的饮食（蛋清<$色氨酸，每天提供1 <$2 g蛋白质/kg; n 14）;增加蛋白质含量的饮食（蛋清<$色氨酸，每天提供1 <$8 g蛋白质/kg; n 14）;标准蛋白质饮食（每天1 <$2 g乳蛋白/kg; n 25）。测试的假设是，接受更多蛋白质饮食的儿童将有更大的非氧化亮氨酸处置率，接受等氮饮食的儿童具有更高的蛋白质质量将有更低的亮氨酸氧化率。在治疗24小时后，使用标准[13 C]亮氨酸稳定同位素示踪技术对儿童进行研究。接受较高蛋白质含量饮食的儿童比接受标准蛋白质含量饮食的儿童具有更高的亮氨酸动力学速率;非氧化亮氨酸处置为170（SD 52）与122（SD 30）mmol亮氨酸/kg/h（P，0 <$01）。与接受标准蛋白质质量饮食的儿童相比，接受增强蛋白质质量饮食的儿童的亮氨酸氧化较少; 34（SD 12）vs. 45（SD 13）mmol亮氨酸/kg/h（P，0 <$05）。接受高蛋白饮食的儿童在开始治疗后24小时增加了6种急性期蛋白中的5种的血清浓度，而接受标准蛋白饮食的儿童则没有。这些数据表明，有更大的全身蛋白质合成，和更有力的急性相反应与高蛋白质含量的饮食。与消瘦、急性感染儿童较高蛋白质摄入量相关的临床益处需要进一步研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The present study compared leucine kinetics and acute-phase-protein concentrations in three groups of marasmic, acutely infected Malawian children fed one of three isoenergetic diets. These were: an enhanced-protein-quality diet (egg-white¿tryptophan, providing 1¿2 g protein/kg per d; n 14); an increased-protein-content diet (egg-white¿tryptophan, providing 1¿8 g protein/kg per d; n 14); a standard-protein diet (1¿2 g milk protein/kg per d; n 25). The hypotheses tested were that children receiving a diet with more protein would have greater rates of non-oxidative leucine disposal and that children receiving an isonitrogenous diet with a higher protein quality would have lower rates of leucine oxidation. The children were studied after 24 h of therapy using standard [13C]leucine stable-isotope tracer techniques. The children receiving the higher-protein-content diet had greater leucine kinetic rates than those receiving the standard-protein-content diet; non-oxidative leucine disposal was 170 (SD 52) v. 122 (SD 30) mmol leucine/kg per h (P,0¿01). Leucine oxidation was less in the children receiving the enhanced-protein-quality diet than in those receiving the standard-protein-quality diet; 34 (SD 12) v. 45 (SD 13) mmol leucine/kg per h (P,0¿05). The children receiving the high-protein-content diet increased their serum concentration for five of six acute-phase proteins 24 h after starting therapy, while those receiving the standard-protein-content diet did not. These data suggest that there was greater whole-body protein synthesis, and a more vigorous acute-phase response associated with the higher-protein-content diet. The clinical benefits associated with a higher protein intake in marasmic, acutely infected children need further study.