THE EFFECT OF DOSE AND COMPOSITION OF DIETARY BEEF ON MUSCLE PROTEIN ANABOLISM

膳食牛肉的剂量和成分对肌肉蛋白合成代谢的影响

• 批准号: 7605414
• 负责人: Douglas Paddon-Jones
• 金额: $ 1.64万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605414
• 关键词: Address; Age; Aging; Amino Acids; Anabolism; Biopsy; Blood specimen; Computer Retrieval of Information on Scientific Projects Database; Daily; Data; Diet; Dietary Intervention; Dietary Proteins; Dose; Eating; Elderly; Exercise; Experimental Designs; Fatty acid glycerol esters; Female; Fibrinogen; Funding; Goals; Grant; Growth; Hormones; Individual; Ingestion; Institution; Intake; Measures; Metabolic; Methodology; Muscle; Muscle Proteins; Numbers; Plasma; Procedures; Protein Biosynthesis; Proteins; Recommendation; Research; Research Personnel; Resources; Skeletal Muscle; Source; Techniques; Tracer; United States National Institutes of Health; beef; male; response; size; stable isotope; volunteer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many individuals lose muscle mass and strength as they get older. One of the factors that may contribute to this loss is changes in the diet. Specifically, elderly individuals may not eat sufficient dietary protein or the protein they do eat may not be as efficiently assimilated into muscle tissue. Previous studies have examined the effect of protein supplements and amino acids on protein synthesis and muscle growth, but there is a lack of data examining the effect of common sources of dietary protein (i.e., beef) on muscle protein synthesis. Hypothesis: Specific hypotheses to be investigated are: 1. In the young, there is a dose response of muscle protein synthesis to the amount of beef ingested. 2. In the elderly, the maximal response of muscle protein synthesis to beef intake is the same as in young, but the submaximal response is diminished. 3. The greater energy content of high fat (25 %) beef will stimulate muscle protein synthesis more effectively than low fat (10 %) beef despite a similar protein content. 4. Prior exercise amplifies muscle protein synthesis following beef ingestion in both young and elderly. Specific Aims and Procedures (summary): The goal of this study is to measure changes in plasma concentrations of amino acids and muscle protein synthesis in young and elderly individuals following ingestion of beef differing in portion size and fat content. Experimental Design (summary): We will study a total of 90 healthy male and female volunteers between the ages of 20 and 50 (young) and 60 and 85 (elderly). We will use established techniques including stable isotope tracer methodology, blood sampling and muscle biopsies to measure changes in plasma hormone and amino acid concentrations and skeletal muscle synthesis. Significance (summary): We propose to investigate the metabolic response to a practical nutritional intervention that will address a number of the mechanisms contributing to muscle protein synthesis and muscle loss during aging. This project will have direct implications for recommendations concerning both the quality and quantity of protein ingested as part of a daily diet and will also provide new information on the relationship between exercise and protein ingestion in the young and elderly.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景： 随着年龄的增长，许多人失去了肌肉和力量。造成这一损失的因素之一可能是饮食的变化。具体地说，老年人可能没有摄入足够的饮食蛋白质，或者他们吃的蛋白质可能没有有效地被肌肉组织同化。以前的研究已经研究了蛋白质补充剂和氨基酸对蛋白质合成和肌肉生长的影响，但缺乏研究常见的饮食蛋白质来源(即牛肉)对肌肉蛋白质合成的影响的数据。 假设： 需要研究的具体假设是： 1.幼鱼肌肉蛋白质合成对肉牛摄入量有剂量效应。 2.老年人肌肉蛋白质合成对牛肉摄入量的最大反应与年轻人相同，但次最大反应减弱。 3.在蛋白质含量相近的情况下，高脂牛肉(25%)的能量含量高于低脂牛肉(10%)，但更有效地刺激肌肉蛋白质的合成。 4.无论是年轻人还是老年人，事先锻炼都能增强摄入牛肉后的肌肉蛋白质合成。 具体目标和程序(摘要)： 这项研究的目的是测量年轻人和老年人在摄入不同份量和脂肪含量的牛肉后血浆氨基酸浓度和肌肉蛋白质合成的变化。 实验设计(摘要)： 我们将研究90名年龄在20岁到50岁(年轻人)和60岁到85岁(老年人)之间的健康男性和女性志愿者。我们将使用成熟的技术，包括稳定同位素示踪方法、血液采样和肌肉活检来测量血浆激素和氨基酸浓度以及骨骼肌合成的变化。 意义(摘要)： 我们建议研究一种实用的营养干预措施的代谢反应，该干预措施将解决在衰老过程中导致肌肉蛋白质合成和肌肉损失的一些机制。该项目将对作为日常饮食一部分的蛋白质摄取的质量和数量方面的建议产生直接影响，并将提供有关年轻人和老年人运动与蛋白质摄取之间关系的新信息。