FLUORESCENT SENSORS FOR DETECTION OF BIOLOGICALLY IMPORTANT ORGANIC COMPOUNDS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们的团队一直参与荧光传感器的制备，用于检测水介质中重要的生物有机化合物。荧光传感器是在目标分子（分析物）存在的情况下产生可见荧光的化合物。这种传感器已被用来直观地追踪细胞内和周围某些分析物的存在。这些研究已被证明是无价的细胞机制的阐明，通过提供实时的信息，一个细胞的环境，以非破坏性的方式。然而，生物上有用的有机化合物传感器落后于金属离子和细胞条件（如pH和pO2）的传感器。制备有机化合物传感器的主要挑战是在细胞的复杂介质中获得对感兴趣的分析物的特异性、高亲和力识别

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our group has been involved in the preparation of fluorescent sensors for detection of biologically important organic compounds in aqueous media. Fluorescent sensors are compounds which produce visible fluorescence in the presence of a target molecule (analyte). Such sensors have been used to visually trace the presence of certain analytes in and around cells. These studies have proven to be invaluable for the elucidation of cellular mechanisms by giving real-time information about the environment of a cell in a non-destructive manner. However, biologically useful sensors for organic compounds have lagged behind those for metal ions and cellular conditions such as pH and pO2. The main challenge in preparing sensors for organic compounds is obtaining specific, high affinity recognition of the analyte of interest in the complex media of the cell