In-Vivo, Wireless Fast-Scan Cyclic Voltammetry System

描述（由申请人提供）：Pinnacle Technology和堪萨斯大学的George Wilson和Michael Johnson教授开发并成功测试了一种原型设备，该设备可以在自由移动的大鼠中无线收集、分析和传输快速扫描循环伏安法（FSCV）数据。原型FSCV系统兼容微电极（直径<10 5m）。该系统可以测量细胞外多巴胺的生理相关水平。在第二阶段的工作中，将开发一个完整的交钥匙无线系统，测试和验证多巴胺。检测其他单胺类神经递质（如去甲肾上腺素和血清素）的能力也将进行测试。这项工作将包括开发具有集成连接器（直径5-50米）的电极、集成刺激、集成微驱动器、优化检测极限、扩展到多个电极（如果可能的话）以及完成商业上可行的采集和分析软件包。分析包将包括与外部刺激、视频或其他相关事件的同步。该系统将进行内部验证，并由独立实验室进行beta测试。拟议产品的开发相对于当前的技术水平是一个非常重要的进步。它将使研究人员能够测量自发的、亚秒级的神经递质释放事件，同时对自由活动的动物进行详细的行为研究。对非拴动物的行为研究提供了正常行为与功能性神经化学之间联系的关键知识，无线快速循环伏安法系统将成为行为、记忆、学习、成瘾机制和睡眠研究人员的重要资产。公共卫生相关性：受异常多巴胺信号严重影响的两种疾病是精神分裂症和帕金森病。估计有290万美国人患有精神分裂症。大约有150万美国人患有帕金森症。根据2000年的估计，每年由精神分裂症和帕金森症引起的直接医疗费用为700亿美元。

DESCRIPTION (provided by applicant): Pinnacle Technology and Professors George Wilson and Michael Johnson of the University of Kansas, have developed, and successfully tested, a prototype device that can collect, analyze and transmit fast-scan cyclic voltammetry (FSCV) data wirelessly in freely moving rats. The prototype FSCV system is compatible with microelectrodes (<10 5m diameter). The system can measure physiologically relevant levels of extracellular dopamine. During this Phase II effort, a complete turnkey, wireless system will be developed, tested, and validated for dopamine. The ability to detect other monoamine neurotransmitters such as norepinephrine, and serotonin will also be tested. This work will include the development of electrodes with integrated connectors (5-50 5m diameter), integrated stimulus, incorporation of a microdrive, optimization of the limit of detection, expansion to multiple electrodes if possible, and completion of a commercially viable acquisition and analysis software package. The analysis package will include synchronization with external stimuli, video or other related events. The system will be validated internally and beta tested by independent laboratories. Development of the proposed product is a highly significant advancement over the current state-of-the art. It will empower researchers to measure spontaneous, sub-second neurotransmitter release events while conducting detailed behavioral studies in freely moving animals. Behavioral studies in untethered animals provide critical knowledge of the link between normal behavior and functional neurochemistry, and a wireless fast cyclic voltammetry system will be a major asset for researchers in behavior, memory, learning, addiction mechanisms and sleep. PUBLIC HEALTH RELEVANCE: Two conditions heavily influenced by abnormal dopamine signaling are schizophrenia and Parkinson's disease. Schizophrenia affects an estimated 2.9 million Americans. Approximately 1.5 million Americans suffer from Parkinson's. Based on year 2000 estimates, the yearly direct health costs due to Schizophrenia and Parkinson's is 70 billion dollars.