BD FACSAria Flow Cytometer

描述（由申请人提供）：本申请的目的是为布朗大学的FACSAria细胞分选仪申请资金。之所以选择FACSAria，是因为它是一种多功能仪器，既可以进行细胞分析，也可以进行细胞分选。这对布朗大学的科学界来说是至关重要的，因为只有一种过时的、有限的细胞分选机可用，而且没有一种机器可以同时分析四种以上的颜色。超过40名博士后研究员、研究生和技术人员依靠现有的FACSCalibur和autoMACS进行分析、分离细胞和细胞分选。新的分选器将配置为最大的光谱范围，细胞分选的灵活性和速度，以及分选细胞的无菌性。这台仪器将极大地造福罗德岛科学界，因为i)复杂的细胞分选和分析对现代生物学至关重要；Ii)目前的细胞分选器不可靠，流式细胞仪的运行已经超出其能力；iii)目前的流式细胞仪设备限制了布朗大学许多NIH资助的研究项目的进展。布朗大学及其附属医院的10个主要用户已经被确定。这10个主要用户预计FACSAria细胞仪总使用量的70%，并作为PI持有NIH每年约500万美元的直接费用拨款。此外，社区中有100多个pi可以作为非主要用户从这个工具中受益。获得最先进的细胞分选器对许多罗德岛研究癌症或病原体免疫反应的研究人员来说至关重要。这将立即影响他们的研究，并有助于促进新药的开发。

DESCRIPTION (provided by applicant): The purpose of this application is to request funding for a FACSAria cell sorter at Brown University. This FACSAria has been chosen because it is a versatile instrument that can do both cell analysis and cell sorting. This is critical for the Brown scientific community because there is only one outdated and limited cell sorter available and no machine that can analyze more than four colors simultaneously. Over 40 post-doctoral fellows, graduate students, and technicians perform studies that are dependent on the current FACSCalibur and autoMACS for analysis, isolation of cells and cell sorting. The new sorter will be configured for maximal spectral range, flexibility and speed of cell sorting, and sterility of sorted cells. This instrument will greatly benefit the Rhode Island scientific community because i) sophisticated cell sorting and analysis is critical for modern biology; ii) the current cell sorter is not reliable and the flow cytometry instruments are already running beyond their capacity; and iii) the current flow cytometry facility limits the progress of many NIH funded research projects at Brown University. A group of ten major users within Brown University and affiliated hospitals has been identified. These ten major users project 70% of the total use of the FACSAria cytometer and hold, as PI, NIH grants with around $5,000,000 in annual direct costs. In addition, there are over 100 PIs in the community who could benefit from this instrument as non-main users. The acquisition of a state of the art cell sorter is critical for many Rhode Island investigators who study cancer or immune response to pathogens. It will impact their research immediately and help to promote the development of new drugs.