AIP1 AND P9 PEPTIDE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们的研究重点是研究HIV是如何利用细胞机械从细胞中发芽的，这些细胞机械通常用于形成晚期内小体的管腔小泡。这一途径中的几种蛋白质是包括艾滋病毒在内的几种逆转录病毒萌发所必需的。该途径中的一种蛋白质是AIP1，它通过病毒中发现的PPXY晚期结构域直接与艾滋病毒和EIAV结合。AIP1也被认为在膜弯曲事件中起着重要作用，因为AIP1除了与也被认为在膜弯曲中起作用的蛋白质结合外，还与晚期内体中发现的特定脂类结合。有关AIP1及其结合伙伴的结构信息将提供对AIP1如何与病毒结合以及AIP1如何在弯曲膜中发挥作用的洞察，这两项都是病毒从细胞中正确萌发的关键。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our research focuses on studying how HIV buds from cells by utilizing cellular machinery normally used in the formation of luminal vesicles of late endosomes. Several proteins in this pathway are required for the budding of several retroviruses, including HIV. One protein in this pathway is AIP1, which binds directly to both HIV and EIAV through a PPXY late domain found in the virus. It is also believed that AIP1 plays an important role in membrane bending events since AIP1 binds to specific lipids found in the late endosome, in addition to binding to proteins which are also believed to function in membrane bending. Structural information on AIP1 and its binding partners will provide insight into how AIP1 binds viruses and how AIP1 could function in bending membranes, both of which are critical for viruses to properly bud from the cell.