STRUCTURES OF PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前正在研究需要同步辐射的多种结构。 这些包括多达42个蛋白亚基的1.1和1.2 MDa复合物（蛋白酶体激活剂复合物），对病毒衣壳组装和动力学重要的HIV-1 GAG衍生结构域的结构，在HIV出芽中起作用的细胞蛋白和复合物，核小体重塑和重组复合物，以及血红素生物合成酶。 在许多情况下，晶体目前已准备好进行数据收集，而在其他情况下，晶体可能在未来几个月/几年内获得。 在某些情况下，我们需要同步辐射，因为衍射本身就很弱，或者因为晶体很小。 其他时候，我们需要高分辨率的可靠性看到复杂的细节，可能会指导未来的努力，在药物开发。 其他时候，我们需要可调辐射，以获得相位信息或本地化差异地图中的异常散射中心。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multiple structures that require synchrotron radiation are currently under investigation. These include 1.1 and 1.2 MDa complexes of up to 42 protein subunits (proteasome activator complexes), structures of HIV-1 Gag-derived domains that are important for viral capsid assembly and dynamics, cellular proteins and complexes that function in HIV budding, nucleosome remodeling and reorganizing complexes, and enzymes of heme biosynthesis. In many cases crystals are currently ready for data collection while in other cases crystals are likely to be obtained within the next few months/years. In some cases we need synchrotron radiation because the diffraction is inherently weak or because crystals are small. Other times we need high resolution to reliability see details of complexes that might guide future effort at drug development. Other times we need tunable radiation in order to obtain phase information or to localize anomalous scattering centers in difference maps.