FACS Core

流式细胞术核心将在该中心现有的基础设施内运行 免疫学和哈罗德·西蒙斯关节炎研究中心支持这项研究 计划调查员的活动。由这些机构提出的个人研究计划 调查人员对试剂和服务有共同的要求。核心将由一个 主任、博士后研究科学家和研究技术员。核心将包括一个 中国人民银行流式细胞仪实验室的样品和设备制备实验室 免疫中心。这个核心的一个目标是从细胞中扩展多色流式细胞术 表面分子与包括细胞内细胞因子和激动酶在内的标记物相匹配。建议数 核心的活动将提供通用和定制的试剂和服务，而不是 否则，对于各个实验室来说，是可用的或经济上可行的。具体的 核心计划包括： 1.抗体标记和资源中心 2.质量控制实验室 3.多参数流式细胞术服务 核心小组与个别项目的主要调查员之间的密切合作将 为了通过使用核心试剂来优化他们的研究生产力 和服务。通过这种方式，将避免冗余，并将在一个 降低了成本。这将允许集中某些技术，例如高速分拣， 多色流式细胞仪和细胞珠阵列，允许质量控制和更多 快速吞吐。

The Flow Cytometry Core will operate within the existing infrastructures of the Center for Immunology and the Harold C. Simmons Arthritis Research Center to support the research activities of Program investigators. The individual research programs proposed by these investigators have common requirements for reagents and services . The Core will consist of a Director, a Postdoctoral Research Scientist and a Research Technician. The Core will include a laboratory for preparation of samples and equipment in the Flow Cytometry Laboratory of the Immunology Center. A goal of this Core is to expand the polychromatic flow cytometry from cell surface lineage molecules to markers that include intracellular cytokines and kinases. The proposed activities of the Core will provide common and custom reagents and services that would not otherwise be available or economically feasible for the individual laboratories. The specific programs of the Core are: 1. Antibody Labeling and Resource Center 2. Quality Control Laboratory 3. Multiparameter Flow Cytometry Service Close collaboration between the Core and the principal investigators for the individual projects will be maintained in order to optimize their research productivity through utilization of Core reagents and services. In this way, redundancy will be avoided and research reagents will be provided at a reduced cost. This will allow for the centralization of certain techniques, such as high speed sorting, polychromatic flow cytometry and cytometric bead arrays, allowing for quality control and more rapid throughput.