Core-Immunology Core

免疫学核心将在协调“宿主防御艾滋病毒相关肺部感染”计划项目中的项目负责人的研究活动方面发挥重要作用。这个核心的使命是提供最先进的技术和程序，用于对免疫系统、肺和其他器官中各种感染模式的细胞和组织进行功能和形态分析。质量控制将是这个核心的首要任务。中心将管理、标准化和执行日常分析程序，以支持正在进行的研究项目，此外，还将开发新的分析方法，以增加与计划项目相关的研究的灵活性。免疫学核心的具体目标是：1) 通过流式细胞术对免疫效应细胞、记忆细胞、干细胞和其他类型的细胞和生物进行单色和多色表型和功能分析；2)通过流式细胞仪对细胞进行分选，以分离、浓缩和克隆有或没有基因转染的细胞；3)用Luminex为基础的多重免疫分析法对生物样品中的炎症介质进行分析；4)用实时定量聚合酶链式反应或逆转录聚合酶链式反应进行基因和基因表达的分析；以及5)通过常规的、 荧光显微镜和共聚焦显微镜。免疫学核心将作为对每个研究部门的人员进行技术培训的基地，以使用核心所采用的设施、程序和分析。核心的专业人员和专家还将咨询数据分析和文档，以支持计划项目的研究。

The Immunology Core will perform a vital role in coordinating the research activities of Project Leaders within the Program Project, "Host Defense Against HIV-related Pulmonary Infections". The mission of this Core is to supply state of the art techniques and procedures for the functional and morphological analysis of cells and tissues in the immune system, lung, and other organs in various models of infections. Quality control will be the first priority of this Core. The Core will administer, standardize, and perform daily analytical procedures to support ongoing research projects, and in addition, will develop novel assays to increase the flexibility of the Program Project-associated research. The specific objectives of the Immunology Core are: 1) to perform single and multiple color phenotypic and functional analysis of immune effector cells, memory cells, stem cells, and other types of cells and organisms by flow cytometry; 2) to perform sorting of cells by flow cytometry for isolation, enrichment, and cloning of cells with or without gene transfection; 3) to conduct analysis of inflammatory mediator profiles in biological samples by Luminex-based multiplex immunoassays; 4) to conduct analysis of gene and gene expression by real-time quantitative PCR or RT-PCR; and 5) to carry out histological, immunohistological, and functional analysis of cells and microbes by conventional, fluorescent, and confocal microscopy. The Immunology Core will serve as a base for technical training of personnel from each research component to use the facilities, procedures, and analyses employed by the Core. Professionals and specialists in the Core will also consult on data analysis and documentation in support of research in the Program Project.