Human Embryonic Stem Cell Core Laboratory

HESC核心自2003年以来一直存在，并已获得并部分表征了21个 NIH批准了hESC系。在此期间，它已经建立了作为hESC有效资源的声誉 高度协作的科学社区中的专业知识。核心将继续协助PPG和 UW/FHCRC调查人员，表征和存储ESC线，使线和衍生品可用于 在MTA的范围内调查人员，培训调查人员，设计hESC培养的新方法 并在未分化和分化的hESCs中鉴定MICO(MI)RNAAs。 在具体目标1中，我们将通过获取和表征以下信息来支持UW/FHCRC的hESC研究 NIH批准的其余七个hESC系，我们将建立一个ES细胞动物园，我们将继续提供 向UW/FHCRC调查人员提供建议和协助。在具体目标2中，我们将发展第二代 HESC培养条件，基于我们的初步观察，4碳脂肪酸丁酸盐可以 在没有条件培养液的情况下支持hESC自我更新。我们还将研究以下方面的影响 在丁酸盐存在下的各种基质。在具体目标3中，我们将描述所有NIH批准的 用于RNA和Micro RNA图谱的hESC系，旨在揭示microRNAs在 HESCS。

The hESC Core has been in existence since 2003, and has acquired and partially characterized 14 of the 21 NIH approved hESC lines. During this time it has established a reputation as an effective resource for hESC expertise within a highly collaborative scientific community. The Core will continue to assist PPG and UW/FHCRC investigators,characterize and store ESC lines, make the lines and derivatives available to investigators within the parameters of the MTA's, train investigators,devise new methods of hESC culture and characterize mico(mi)RNAsin undifferentiated and differentiated hESCs. In Specific Aim 1 we will support hESC research at UW/FHCRC by acquiring and characterizing the remaining seven NIH approved hESC lines, we will build an ES cell zoo,and we will continue to provide advice and assistance to UW/FHCRC investigators. In Specific Aim 2 we will develop second generation hESC culture conditions, building upon our initial observation that the 4 carbon fatty acid, butyrate,can support hESC self renewal in the absence of conditioned medium. We will also examine the effects of various matrices in the presence of butyrate. In Specific Aim 3 we will characterize all the NIH-Approved hESC lines for RNA and micro RNA Profiles, studies designed to reveal the function of microRNAs in hESCs.