Shared Zeiss 510META Confocal Microscope

描述（由申请人提供）：本申请是为了购买一台最先进的蔡司共聚焦显微镜，该显微镜将被安置在布朗大学分子医学实验室（LMM）的新生物医学研究大楼内。该设备将为15名NIH资助的教师提供服务。使用该范围的实验室成员包括学生（研究生，医学，MD-PhD候选人和本科生）博士后博士研究员，临床部门的医学研究员和技术人员，总共有60多个潜在用户。新的LMM位于布朗附属医院的研究大楼附近。LMM设有三个基础科学和医学院部门（病理学，分子药理学，生理学和生物技术，分子和细胞生物学和生物化学部门）。此外，还有三个中心位于新大楼，包括遗传学和基因组学中心和一个新的环境健康和技术中心。该新设备的主要用户群包括LMM的所有部门和中心。新成像将应用于的研究项目涵盖衰老和阿尔茨海默病的分子基础，新兴病原体和毒理学。大多数待成像的生物体需要温度控制以实现生理稳态。在这种新的环境中，医学和基础科学专家团队之间的跨学科交流机会是巨大的。活细胞的分子成像已经成为一种强大的工具，没有它，大多数基础研究问题都无法得到明确的解决。为了实时记录细胞/分子动力学，需要专门的设备，如本文提出的共聚焦显微镜。由Meta和多跟踪仪扫描头和软件选项提供的多光谱解混允许在同一样本中随时间推移成像多达8个荧光标记。通过电动z载物台，可以在5个维度（分子、3D结构和时间推移）中捕获该成像。这种专用显微镜必须放置在培养/创建活标本的实验室附近，因为运输此类标本会产生人工制品。目前，在这座新建筑中只有一台过时的共焦，一台1993年购买的Zeiss 410。这个共享仪器的建议是增加一个新的显微镜的成像设施在这个新的显微镜将管理下现有的LeDuc生物医学成像设施和共聚焦用户委员会。相关性：该共聚焦显微镜的主要用户组包括一系列临床，转化和基础科学NIH资助的研究人员，跨越一系列部门和学科。它们的研究应用解决了对公共卫生有重要影响的流行疾病的基本问题。

DESCRIPTION (provided by applicant): This application is for the purchase of a state-of-the-art Zeiss confocal microscope to be housed in a new Biomedical Research building, the Laboratory of Molecular Medicine (LMM) at Brown University. This equipment will serve 15 NIH-funded faculty. Members of laboratories using the scope include students (graduate, medical, MD-PhD candidates, and undergraduates) post-doctoral PhD fellows, medical fellows from clinical departments, and technical staff, for a total of over 60 potential users. The new LMM is located near research buildings of the Brown affiliated hospitals. LMM houses three basic science and medical school departments (Departments of Pathology, of Molecular Pharmacology, Physiology and Biotechnology, and Molecular and Cellular Biology and Biochemistry). In addition, there are three Centers located in the new building, including Center for Genetics and Genomics and a new Center for Environmental Health and Technology. Major User Group for this new equipment includes all departments and Centers in LMM. The research projects for which the new imaging will be applied span from the molecular basis of aging and Alzheimer's disease, to emerging pathogens, and toxicology. Most organisms to be imaged require temperature control for physiological homeostasis. The opportunities for cross-disciplinary fertilization among teams of medical and basic science experts are immense in this new environment. Molecular imaging of live cells has become such a powerful tool that most basic research questions cannot be addressed definitively without it. For live recording of cellular/molecular dynamics, specialized equipment such as the confocal microscope proposed here is necessary. Multispectral un-mixing provided by the META and multitracker scan head and software options allow up to eight fluorescent markers to be imaged in the same specimen over time. With the motorized z-stage, this imaging is captured in 5 dimensions (molecular, 3D structural and time lapse). This specialized microscope must be housed in close proximity to the laboratories where the living specimens are cultured/created, as transport of such specimens produces artifacts. At present, there is only one out-dated confocal in this new building, a Zeiss410 purchased in 1993. This shared instrumentation proposal is to add a new microscope to the imaging facility at This new microscope will be managed under the existing LeDuc Biomedical Imaging Facility and the Confocal Users Committee. Relevance: The Major Users Group for this proposed confocal microscope includes a range of clinical, translational and basic science NIH-funded investigators spanning a range of departments and disciplines. Their research applications address fundamental questions regarding prevalent disease with important impacts on public health.