MALDI TOF/TOF Mass Spectrometer

描述（由申请人提供）：我们建议购买MALDI TOF/TOF质谱仪，这将为威斯康星大学麦迪逊分校的健康相关研究提供重要支持。将受益的具体研究项目包括：1)设计非天然低聚物（折叠物）以阻止疾病相关的蛋白质-蛋白质相互作用；2)作为植入骨科医疗器械的组织工程基质或涂层的生物材料的开发；3)线虫中神经肽的鉴定，这是神经系统功能的简单模型；4)设计破坏细菌群体感应的小分子，这可以作为基础生物学研究的工具，并导致新的抗菌剂；5)绘制水果和其他食物中发现的多酚（单宁）与细胞培养和体外炎症、细菌粘附和氧化模型中的生物活性之间的关系；6)与核糖体生物合成同时发生的蛋白质折叠的阐明；7)设计新的聚合物，模拟宿主防御肽的选择性抗菌活性。该仪器将是华盛顿大学化学系质谱设备的重要补充，因为它将允许通过碎片化进行常规结构测定（例如，肽、肽类似物和聚合物的测序）。化学MS设施在西澳大学校园内是独一无二的，允许学生和其他研究人员24小时访问，这对于上述项目的快速进展至关重要。因此，所要求的仪器所提供的能力将构成对美国一个主要研究机构的科学基础设施的重要补充。

DESCRIPTION (provided by applicant): We propose to acquire a MALDI TOF/TOF mass spectrometer, which will provide crucial support for health-related research at UW-Madison. Specific research projects that will benefit include: 1) the design of unnatural oligomers (foldamers) to block disease-associated protein-protein interactions; 2) the development of biomaterials that function as tissue engineering substrates or coatings for implanted orthopedic medical devices; 3) the identification of neuropeptides in the nematode, a simple model for nervous system function; 4) the design of small molecules that disrupt bacterial quorum sensing, which can serve as tools for basic biological research and as leads to new antibacterial agents; 5) the mapping of relationships between polyphenols (tannins) found in fruits and other foods and bioactivity in cell culture and in vitro models of inflammation, bacterial adherence and oxidation; 6) the elucidation of protein folding that occurs concomitantly with ribosomal biosynthesis; 7) the design of new polymers that mimic the selective antimicrobial activity of host-defense peptides. The proposed instrument will be a crucial addition to the MS facility in the UW Chemistry Department because it will allow routine structure determination via fragmentation (e.g., sequencing of peptides, peptide analogues and polymers). The Chemistry MS facility is unique on the UW campus in allowing 24-hour access to students and other researchers, which is essential for rapid progress in the projects described above. Therefore, the capabilities provided by the requested instrument would constitute an important addition to the scientific infrastructure at a major US research institution.