Core--Biochemistry

生物化学核心实验室将为项目内的个别项目提供生化分析服务以及组织学和免疫组织化学分析。生化分析包括IL-6、il -1 β、TNFalpha、tgf β、ET-1、微量白蛋白、去甲肾上腺素、组织总蛋白、血浆纤溶活性、血浆肾素、皮质酮、纤维连接蛋白、电解质、肌酐、8-异前列腺素、cGMP、6-酮PGF（1 α）、PGE2、PGF（2 α）、TxB2在小鼠和大鼠尿液、血浆和/或组织提取物中的测量。技术将包括放射免疫测定、放射酶测定、ELISA和离子选择性

The Biochemistry Core laboratory will provide biochemical assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include IL-6, IL-1beta, TNFalpha, TGFbeta, ET-1, microalbumin, norepinephrine, tissue total protein, plasma fibrinolytic activity, plasma renin, corticosterone, fibronectin, electrolytes, creatinine, 8- isoprostane, cGMP, 6-keto PGF(1alpha), PGE2, PGF(2alpha), TxB2 measurements in urine, plasma, and/or tissue extractsfrom mice and rats. Techniques will include radioimmunoassay, radioenzymatic assay, ELISA, and ion-selective electrodes. Histology and immunohistochemical analyses will provide hematoxylin & eosin (H&E), periodic acid-Schiff (PAS), Sirius red, blue trichrome, and specific immunohistochemical staining analyses for four of the projects in the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.