Immunology Core

免疫学核心的主要目标是提供流式细胞仪技术支持和专业知识 促进艾滋病毒/艾滋病研究方面的研究。除了通用技术，酷睿还将提供独特的 为分析T细胞亚群数量变化的研究提供服务和专业知识。为研究提供支持 正在完成-由CFAR调查员完成，具体目标是：(T)提供机械和技术 支持需要使用流式细胞仪分析细胞标志物的研究；(2)提供工具和服务 以及量化T细胞亚群表达不同T细胞受体(TCR)V区和 通过流式细胞术对抗原特异性T细胞亚群进行定量；以及(3)提供机械和技术 支持需要用流式细胞术对潜在感染细胞进行分类的研究。AIM 2将包括 主要组织相容性复合体(MHC)/多肽染色分析TCR V区表达 多聚体和抗原刺激后T细胞胞内细胞因子的表达。为了支持AIMS 2， CORE将维护一组针对不同TCR V区域的单抗，并提供高质量 对这些试剂的使用进行控制。此外，核心将生产和监测MHC II类/多肽 CFAR调查人员用于分析与HIV感染相关的抗原特异性T细胞的多聚体 个人。免疫学核心的支持将使这些试剂的使用和分析性能得以实现 以一致和高质量的方式基于流式细胞术。

The major goal of the Immunology Core is to provide flow cytometry technical support and expertise to facilitate studies in HIV/AIDS research. In addition to general technology, the Core will provide unique services and expertise for studies that analyze quantitative changes in T cell subsets. In the support of studies being done-by CFAR investigators_ specifi_ Aims of the eore inelude: (t) to provide machinery and technical support for studies requiring the analysis of cell markers using flow cytometry; (2) to provide tools and services and expertise for studies that quantitate T cell subsets expressing different T cell receptor (TCR) V regions and that quantitate antigen-specific T cell subsets by flow cytometry; and (3) to provide machinery and technical support for studies requiring the sorting of potentially infected cells by flow cytometry. Aim 2 will include analyses of TCR V region expression, staining with major histocompatibility complex (MHC)/peptide multimers, and T cell intracellular cytokine expression after stimulation with antigen. To support Aims 2, the Core will maintain a panel of monoclonal antibodies directed to different TCR V regions and provide quality control for the use of these reagents. In addition, the Core will produce and monitor MHC class II/peptide multimers for CFAR investigators to use for analysis of antigen-specific T cells relevant to HIV infected individuals. Support of the Immunology Core will allow the use of these reagents and performance of analyses based on flow cytometry in a consistent and high quality manner.