Topical Microbicide Against SIV and Chlamydia

描述（由申请人提供）：本申请的主要目的是测试一种潜在的杀微生物剂预防艾滋病毒传播给妇女的功效。迫切需要这样一种有效的药物来对付全球妇女新感染艾滋病毒人数的显著上升。基于有希望的体外初步结果，本应用程序的具体目的是在体内测试单月月酸甘油（GML）预防猴免疫缺陷病毒（SIV）在恒河猴阴道内传播的功效，恒河猴是HIV性粘膜传播的高度相关的非人灵长类动物模型。治疗或对照的猴子将被感染，活检和尸检标本将通过免疫组织化学和原位杂交方法和血液中的病毒载量进行分析，以评估GML在预防传播或改变病毒在宫颈阴道和阴道中的复制方面的功效

DESCRIPTION (provided by applicant): This application's major objective is to test the efficacy of a potential microbicidal agent to prevent transmission of HIV to women. Such an effective agent is urgently needed to combat the predominant rise globally of new HIV infections in women. Based on promising preliminary results in vitro, the specific aim of this application is to test in vivo the efficacy of glycerol monolaurate (GML) to prevent intravaginal transmission of simian immunodeficiency virus (SIV) to rhesus macaques, a highly relevant nonhuman primate model of sexual mucosal transmission of HIV. Treated or control monkeys will be infected and biopsy and necropsy specimens will analyzed by immunohistochemical and in situ hybridization methods and viral loads in blood to assess the efficacy of GML in preventing transmission or altering viral replication in cervicovaginal and lymphatic tissues.