CORE--MOLECULAR GENETICS

描述（由申请人提供）：本核心的总体目的是为中心成员提供服务，科学知识和技术专长，以扩大分子生物学方法在其研究中的应用。该设施核心的具体目标是：1)为研究项目的发展提供支持，这些项目可以利用最先进的DNA和RNA分析资源，为会员提供服务；2)为研究宿主对环境化合物反应的分子机制的新项目和新方法的构想和实施提供核心分子生物学资源。该核心为支持中心环境健康研究中基于dna的分子方法提供了资源。核心为DNA测序、聚合酶链反应、DNA片段分析、DNA微阵列方法、寡核苷酸设计和有限的生物信息学支持提供集中的设施。DNA测序实验室提供最新的自动DNA测序方法，主要由RWJMS新泽西癌症研究所提供支持，并得到本中心的额外支持。微阵列服务是自上次审查以来新增的，新泽西癌症研究所新成立的实验室将提供这些服务。通过与外部商业供应商签订合同，通过订购合成寡核苷酸的外包机制提供合成DNA。中心中心强调基本分子生物学技术的教育和教学培训，并主办关于该设施提供的服务的技术研讨会。

Description (provided by applicant): The overall purpose of this core is to provide members of the Center with service, scientific knowledge, and technical expertise to expand the application of molecular biological approaches in their research. The specific objectives of this facility core are, 1) to provide support for the development of research programs that can capitalize on state-of-the-art DNA and RNA analysis resources provided as a service to members; and 2) to serve as a central molecular biology resource for the conception and execution of new projects and approaches for examining the molecular mechanisms of host responses to environmental compounds. This core provides resources to support DNA-based molecular approaches in environmental health research at the Center. The core provides centralized facilities for DNA sequencing, polymerase chain reaction, DNA fragment analysis, DNA microarray methods, oligonucleotide design, and limited bioinformatics support. The DNA sequencing laboratory provides up-to-date methods for automated DNA sequencing and is largely supported by the Cancer Institute of New Jersey at RWJMS with additional support from this Center grant. The microarray service is a new addition since the last review, and a newly established laboratory in the Cancer Institute of New Jersey will provide these services. Synthetic DNA is provided via an outsourcing mechanism for ordering synthetic oligonucleotides through a contract with an outside commercial vendor. The central core emphasizes educational and instructional training in basic molecular biology techniques and sponsors technical seminars on the services provided by the facility.