Flow Cytometry And Functional Immunology Research

流式细胞术的目的和功能免疫学研究的核心是提供一个集中的 技术专长和主要设备的资源，以支持和加强试验设计和 开展需要应用流式细胞术的艾滋病毒发病机制的基础和临床研究 表型分析、细胞分选和/或特定免疫细胞功能的评估。首要目标是 提供智力环境和物质资源，使初级调查人员能够应用心流 将细胞学技术应用于他们的项目，并鼓励老牌研究人员启动创新试验 学习。为实现这些目标，通过核心主任协理提供专家咨询 董事、技术监督员；选择功能互补的主要仪器； 通过专门的技术人员操作员可以使用设备；服务是通过充电提供的 基础；对生成的数据进行分析、简化并绘制图表；并为试点和 探索性研究项目通过提供研究试剂和仪器时间。恒虚警 资源使核心能够支持广泛的艾滋病毒研究，包括以下方面的基本项目 疫苗和基因临床前开发的病毒调控和致病机制 原发感染、机会性感染、神经生物学、免疫和免疫的治疗和临床研究 疫苗和HAART的病毒反应。 为了维护我们的流式细胞仪设备以满足圣地亚哥学术研究界的需求， 已经整合了几个资金来源，以提供合作支持：加州大学圣地亚哥分校艾滋病中心 研究(CFAR)、退伍军人事务部(RCAHI)和退伍军人事务部 医学研究基金会(VMRF)。除了加州大学圣地亚哥分校和退伍军人事务部的主要调查人员 医学中心，来自斯克里普斯研究所、索尔克研究所、拉霍亚研究所的研究人员 过敏和免疫学研究所和伯纳姆研究所也利用我们的中心提供的资源 设施。当地对流式细胞仪技术的使用继续增长和多样化，核心设施努力 以跟上不断增长的需求。CFAR奖仍然是这一努力中不可或缺的组成部分。 流式细胞术在生物分子研究的几个领域的应用 在临床研究中的应用，使得持续的CFAR支持对于提供基础设施至关重要 利用艾滋病毒研究领域的新科学机遇。

The purpose of the Flow Cytometry and Functional Immunology Research Core is to provide a centralized resource of technical expertise and major equipment to support and enhance the experimental design and execution of basic and clinical research in HIV pathogenesis that require application of flow cytometric phenotypic analysis, cell sorting, and/or evaluation of specific immune cell functions. A prime goal is to provide the intellectual environment and the material resources to enable junior investigators to apply flow cytometry technology to their projects and to encourage established investigators to initiate innovative pilot studies. To achieve these objectives, expert consultation is provided through the Core Director, Associate Directors, and technical supervisor; major instruments are selected for complementary functions; equipment use is accessible through dedicated technician operators; services are provided on a recharge basis; generated data are analyzed, reduced, and diagramed; and fiscal support is given to pilot and exploratory research projects through the provision of research reagents and instrument time. CFAR resources have enabled the Core to support a wide array of HIV research encompassing basic projects on viral regulation and mechanisms of pathogenesis to preclinical development of vaccines and gene therapies, and clinical studies of primary infection, opportunistic infections, neurobiology, and immune and viral responses to vaccines and HAART. To maintain our flow cytometry facility to meet the needs of the San Diego academic research community, several funding sources have been integrated for cooperative support: the UCSD Center for AIDS Research (CFAR), the VA Research Center for AIDS and HIV Infection (RCAHI), and the Veterans Affairs Medical Research Foundation (VMRF). In addition to principal investigators based at UCSD and the VA Medical Center, investigators from The Scripps Research Institute, the Salk Institute, the La Jolla Institute of Allergy and Immunology, and the Burnham Institute also utilize the resources provided by our central facility. Local use of flow cytometric technology continues to grow and diversify, and the Core facility strives to keep up with the growing demand. The CFAR award remains an integral component in this endeavor. Application of flow cytometry to several areas of biomolecular research, in addition to expanded applications in clinical research, makes continued CFAR support crucial to providing the infrastructure to take advantage of new scientific opportunities in HIV research.