Cell and Tissue Imaging Core Facility

癌症中心“细胞和组织成像核心设施”(以前称为“共焦核心设施”) 正在进入运营的第六个年头。它的使命，由癌症中心执行委员会制定， 是提供先进的细胞和组织成像技术、服务和科学咨询，以促进 科学互动和提高癌症中心研究人员的科学生产力。该设施是 最初是作为与Doheny Eye Institute的伙伴关系开发的；合作伙伴研究所联合购买了 第5代共聚焦显微镜，并将该仪器安装在现有的电子显微镜成像设备中 与癌症中心相邻的设施。该设施由癌症中心和多赫尼联合管理 眼科研究所。这个共享设施的2000平方英尺的实验室空间是由Doheny Eye提供的 研究所不向癌症中心支付任何费用。因此，该设施50%由癌症中心使用，50%由癌症中心使用 多尼眼科研究所。本提案中要求的所有资金都用于与癌症直接相关的费用 中心50%的设施使用率。 这个共享资源机构的主要用户是拥有同行评审的癌症中心调查人员， 受资助的项目。该设施也向需要其设施的其他癌症中心成员开放 开发试点项目数据。自成立以来，该设施为癌症中心提供了 会员可轻松获得“最先进”的细胞和组织成像设备和技术支持。 白天共焦显微镜的使用率仍然很高(90%的可用时间利用率)；大约 其中50%的使用来自癌症中心成员(与50：50的使用安排一致 多尼眼科研究所)。重要的是，癌症中心可以使用的成像设备和服务的范围 成员已扩展(超越激光扫描共焦/多光子显微镜)，包括完全访问 透射电子显微镜、扫描电子显微镜、数字光和荧光 显微镜、荧光和明场激光捕获显微切割、薄层切片、冷冻切片和 嵌入程序和计算机辅助图形学。我们正在不断升级我们的成像软件 并建议和指导癌症中心的研究人员使用新的方法和方案来增强 它们的细胞和组织成像能力。

The Cancer Center "Cell and Tissue Imaging Core Facility" (previously the "Confocal Core Facility") is entering its sixth year of operation. Its mission, as developed by the Cancer Center Executive Committee, is to provide advanced cell and tissue imaging technology, services, and scientific consultation that facilitate scientific interaction and enhance scientific productivity of Cancer Center investigators. The Facility was originally developed as a partnership with the Doheny Eye Institute; the partner institutes jointly purchased a 5th generation confocal microscope and housed the instrument in an existing electron microscopy imaging facility adjacent to the Cancer Center. The Facility is jointly managed by the Cancer Center and the Doheny Eye Institute. The 2,000 square foot laboratory space for this shared facility is provided by the Doheny Eye Institute at no cost to the Cancer Center. The Facility is therefore used 50% by the Cancer Center and 50% by Doheny Eye Institute. All funds requested in this proposal are for costs directly related to the Cancer Center's 50% use of the facility. The primary users of this shared resource facility are Cancer Center investigators with peerreviewed, funded projects. The Facility is also open to other Cancer Center members who need its facilities to develop pilot project data. Since its establishment, the Facility has provided the Cancer Center membership with easy access to "state-of-the-art" cell and tissue imaging equipment and technical support. Daytime usage of the confocal microscope remains heavy (90% utilization of available hours); approximately 50% of this usage comes from Cancer Center members (consistent with the 50:50 use arrangement with Doheny Eye Institute). Importantly, the range of imaging equipment and services available to Cancer Center members has expanded (beyond laser scanning confocal/multiphoton microscopy) to include full access to transmission electron microscopy (TEM), scanning electron microscopy (SEM), digital light and fluorescence microscopy, fluorescence and bright field laser capture microdissection, thin sectioning, cryo-sectioning and embedding procedures, and computer aided graphics. We are continuously upgrading our imaging software and advising and instructing Cancer Center investigators in novel methodologies and protocols to enhance their cell and tissue imaging capability.