Direct sampling time resolved detector

描述(由申请人提供)：该提案描述了一种新的频域时间分辨测量方法的开发。应用领域包括荧光显微镜、荧光光谱学和光子迁移。该方案是一种将时间域方法的灵敏度与频域的速度和动态范围相结合的混合方法。它比目前所有的频域荧光和光子迁移技术都具有无可比拟的优势，在微光下的灵敏度和信噪比方面与时间相关的单光子计数法相竞争，同时仍然能够处理高光子计数率。它将使广泛的学科受益，包括荧光寿命成像、荧光共振能量转移、光子迁移、高通量筛选和流式细胞术。这项提议将对从癌症生物学、药物开发、细胞生物学、组织成像和非侵入性诊断在内的一系列生物医学应用产生广泛的影响。这项提议描述了一种新的时间分辨检测方法的发展，该方法比以前的方法更灵敏，适用范围更广。它将有助于新生儿护理、中风诊断、蛋白质动力学研究、药物开发和癌症诊断等领域。

DESCRIPTION (provided by applicant): The proposal describes the development of a novel method for frequency domain time resolved measurements. Application areas include fluorescence microscopy, fluorescence spectroscopy, and photon migration. The proposal is a hybrid approach that combines the sensitivity of time domain methods with the speed and dynamic range of the frequency domain. It has unqualified superiority over all current frequency domain fluorescence and photon migration techniques and rivals Time Correlated Single Photon Counting in sensitivity and signal to noise at low light levels while still be able to process high photon count rates. It will benefit a broad range of disciplines, including fluorescence lifetime imaging, fluorescence resonance energy transfer, photon migration, high throughput screening, and flow cytometry. This proposal will have broad impact in a range of biomedical applications from cancer biology, pharmaceutical development, cell biology, tissue imaging, and noninvasive diagnosis. This proposal describes the development of a new time resolved detection method which is significantly more sensitive and widely applicable than previous approaches. It will help areas such as neonatal care, stroke diagnosis, the study of protein dynamics, drug development and cancer diagnosis.