Development of an In Situ Anhydrobiotic Tissue Culture Process

描述（由申请人提供）：Cell Preservation Services，Inc.（CPSI）是一家处于早期阶段的生物技术公司，打算开发一种独特的技术，以目前尚不存在的方式促进人类细胞培养物的时间管理和全球分销。根据该SBIR，CPSI打算开发一种即用型预接种细胞培养产品“DryPlates”，用于多种细胞类型，包括标准细胞系、原代细胞（如角质形成细胞或肝细胞）和祖细胞。该技术将允许用户按需访问细胞以进行高通量使用，同时消除了在使用前对细胞进行大量处理和培养的需要。DryPlates”产品的设计以方便的形式提供了生物制品的长期稳定的非冷冻储存，易于与当前方法集成。CPSI打算研究脱水生物过程的细胞和分子组成部分，以开发一种生产DryPlates的技术和方法，以满足工业的需求，包括药物发现，生物防御和干细胞研究。公共卫生相关性：该提案旨在开发一种独特的创新技术，用于制药和生物医学行业的人类细胞培养物的全球分销。

DESCRIPTION (provided by applicant): Cell Preservation Services, Inc. (CPSI) is an early stage biotechnology company that intends to develop a unique technology that will facilitate both the time- management and worldwide distribution of human cell cultures in a manner that currently does not exist. Under this SBIR, CPSI intends to develop a ready-to- use pre-seeded cell culture product, DryPlates", for several cell types, including standard cell lines, primary cells, such as keratinocytes or hepatocytes, and progenitor cells. This technology will allow users on-demand access to cells for high-throughput usage while eliminating the need for extensive handling and culturing of cells prior to utilization. The design of DryPlates" products provides for the long-term stable non-frozen storage of biologicals in a convenient format, easily integratable with current methods. CPSI intends to investigate the cellular and molecular components of the anhydrobiotic process to develop a technology and method of production of DryPlates" to meet the needs of industry, including pharmaceutical drug discovery, biodefense and stem cell research. PUBLIC HEALTH RELEVANCE: This proposal is designed to develop a unique and innovative technology for the worldwide distribution of human cell cultures to be used in the pharmaceutical and biomedical industries.