ANIMAL CORE

Animal Core B的主要目的是继续提供SAMP 1/YitFc（SAMP）小鼠，用于组成该项目的各个项目。动物中心将在统一的环境条件下，将SAMP育种群体、其衍生物（包括SAMP同源系）以及亲本对照（AKR）群体保存在共同的集中设施中。上述小鼠的饲养对于该计划的成功至关重要，特别是鉴于有证据表明环境因素，如特定肠道细菌的定植，可能影响疾病的存在或病程。动物生产的集中化还通过消除多余的繁殖群体来降低成本，并通过确保实验动物的有效利用来最终减少实验所需的动物总数。 五个项目中的每一个。此外，动物核心协调实验小鼠的分布，使五个项目中的每一个项目都能进行最佳的沟通和合作。第二个具体目标是继续为研究人员提供SAMP小鼠肠道炎症和其他形态学参数的集中组织学分析，以监测表型和组织病理学随时间的变化。动物核心的这一组成部分的主要优势在于，经验丰富的GI病理学家（CA Moskaluk）可持续对SAMP组织进行定期组织学分析，并就该模型中疾病的病理学特征向PPG研究者提供持续的咨询服务。第三个具体目标是继续作为一种资源，用于向小鼠提供特异性引入SAMP背景品系的基因突变，这将有助于 项目中的多个项目。动物核心的这一组成部分与UVA的小鼠遗传学核心设施密切互动，由Marcia McDuffie博士（项目2的主要研究者）指导。使用快速同源技术，可以在三到四代中完成这些诱导突变到适当背景中的转移。Theresa Pizarro博士将继续指导项目动物核心的整体活动，由核心总监、核心顾问（CA Moskaluk）和项目PI（F Cominelli）组成的委员会将定期开会，以解决常规质量控制和优先级问题。此外，由咨询委员会成员组成的监督委员会将每年审查核心小组的总体运作情况。

The primary aim of the Animal Core B is to continue to provide SAMP1/YitFc (SAMP) mice for use in each of the individual projects comprising the Program Project. The Animal Core will maintain the SAMP breeding colony, its derivations including SAMP congenic lines, as well as the parental control (AKR) colony in a common centralized facility under uniform environmental conditions. Housing of the aforementioned mice is critical to the success of the program, particularly in the light of evidence that environmental factors, such as colonization of specific commensal bacteria, can affect the presence or course of disease. Centralization of animal production also reduces costs, by eliminating redundant breeding colonies, and ultimately reduces the overall number of animals required for experimentation by ensuring efficient utilization of experimental mice by each of the five projects. In addition, coordination of the distribution of experimental mice by the Animal Core allows optimal communication and collaboration between each of the five projects. A second specific aim is to continue to provide investigators with centralized histological analyses of intestinal inflammation and other morphological parameters in SAMP mice, to monitor phenotypic and histopathological changes over time. A major strength of this component of the Animal Core is the continued availability of an experienced Gl pathologist (CA Moskaluk) to perform periodic histological analyses of SAMP tissues and to provide ongoing consultative services to PPG investigators regarding the pathological features of disease in this model. The third specific aim is to continue to serve as a resource for providing mice with specifically introduced genetic mutations onto the SAMP background strain that will be useful to multiple projects within the program. This component of the Animal Core interacts closely with the Mouse Genetics Core Facility at UVA, directed by Dr. Marcia McDuffie (Principal Investigator of Project 2). Using speed congenic technology, transfer of these induced mutations into the appropriate background can be accomplished in three to four generations. Dr. Theresa Pizarro will continue to direct the overall activities of the Program Project Animal Core, and a committee consisting of the Core Director, Core Consultant (CA Moskaluk), and the PI of the Program Project (F Cominelli), will meet on a regular basis to address routine quality control and prioritization issues. In addition, an oversight committee consisting of members of the Advisory Board will review the overall operation of the Core on a yearly basis.