DEVELOPMENT OF CORE SERVICE FOR SIV CELLULAR IMMUNE ASSAYS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞免疫学核心是由Marcelo黑田博士领导的免疫学部门的一部分。 核心由一名实验室经理和一名医学研究专家组成，他们根据要求为核心和附属研究者的特定研究项目提供免疫学服务。 目前的服务包括在标准化程序下进行样品制备以进行最佳测定分析、ELISPOT测定的规划和执行、数据处理和呈现、细胞内细胞因子染色、Mamu-A*01、Mamu-A*02和Mamu-DRW*201四聚体构建以及MHC分型。 我们将继续使用MabTech ELISPOT系统以及U-Cytech系统，具体取决于特定的项目和合作者。 在大多数情况下，我们继续使用Zellnet咨询服务来记录和分析ELISPOT结果。我们继续为核心和附属研究者提供ELISPOT服务，分析了47个动物时间点对多种重组抗原的反应。 细胞免疫学中心还免费为研究人员提供MHC分型服务。在这一年中，在TNPRC分配到不同项目的100多只恒河猴进行了测试，以确定是否存在特定的恒河猴MHC-I和II基因。Core已实施PCR检测，可准确确定Mamu A01、Mamu A02和Mamu-DRw 201分子的存在。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cellular Immunology Core is a part of the Immunology Division headed by Dr. Marcelo Kuroda. The core is staffed by one laboratory manager and one Medical Research Specialist who provide immunology services to specific research projects of core and affiliate investigators as requested. Current services include sample preparation under standardized procedures for optimal assay analysis, planning and performance of ELISPOT assays, data processing and presentation, intracellular cytokine staining, Mamu-A*01, Mamu-A*02 and Mamu-DRW*201 tetramer construction and MHC typing. We are continuing to use the MabTech ELISPOT system as well as the U-Cytech system depending on the particular project and collaborators. For the most part we have continued to use the Zellnet Consulting service for recording and analyzing the ELISPOT results. We continue providing ELISPOT service to the core and affiliate investigators resulting in analysis of 47 animal time-points for responses to up to multiple recombinant antigens. The Cellular Immunology Core also provides MHC typing service at free cost to investigators. During this year, more than one hundred Rhesus monkeys assigned to different projects at the TNPRC were tested for the presence of a particular Rhesus monkey MHC-I and II genes. The Core has implemented PCR test that accurately determined the presence Mamu A01, Mamu A02 and Mamu-DRw201 molecules.