FIBRONECTIN-INTEGRIN BINDING & POST MENSTRUAL REPAIR IN THE MACAQUE ENDOMETRIUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的目标是更好地了解正常月经和月经失调，包括月经过多和突破性出血。 月经是子宫内膜破坏和再生的周期性过程，由月经周期结束时孕酮（P）的急性撤回引发。 几项研究表明，停药可引发炎症样反应，包括白细胞介素-8（IL 8）和单核细胞趋化蛋白-1（MCP 1）的上调，从而刺激月经期间中性粒细胞、单核细胞和巨噬细胞的募集。 在月经后期和月经周期的子宫内膜修复期，细胞外基质蛋白纤连蛋白在子宫内膜的最高功能区也显著上调。 我们推测纤连蛋白-整合素结合可能在调节炎症反应中发挥作用，并在月经出血停止期间进一步发挥重要作用。 本研究有两个具体的目的：目的1是表征纤维连接蛋白，整合素和炎性趋化因子受体在猕猴月经期子宫内膜的表达模式。 目的2是确定纤连蛋白-整合素阻断剂对月经和子宫内膜修复相关基因调控的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to better understand normal menstruation and menstrual disorders including menorrhagia and breakthrough bleeding. Menstruation is a cyclical process of endometrial destruction and regeneration, initiated by an acute withdrawal of progesterone (P) at the end of the menstrual cycle. Several studies indicate that P withdrawal triggers an inflammation-like response that includes up-regulation of interleukin-8 (IL 8) and monocyte chemotactic protein-1 (MCP 1) that can stimulate the recruitment of neutrophils, monocytes and macrophages during menses. The extracellular matrix protein fibronectin is also dramatically up-regulated in the uppermost functionalis zones of the endometrium during late menstruation and the endometrial repair phase of the menstrual cycle. We hypothesize that fibronectin-integrin binding may play a role in regulating the inflammatory response and further have essential actions during the cessation of menstrual bleeding. This study has two specific aims: Aim 1 is to characterize the pattern of fibronectin, integrin and inflammatory chemokine receptor expression in the macaque endometrium during menses. Aim 2 is to determine the effect of fibronectin-integrin blockade on the regulation of genes associated with menstruation and endometrial repair.