CORE C: HISTOPATHOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Core C为干细胞Cobre和其他MMCRI研究人员提供专业的组织学服务。服务包括组织固定、包埋、冰冻、石蜡或塑料包埋标本切片，以及某些染色程序。组织学染色包括苏木精-伊红、三色染色、von Kossa染色、地衣染色、van Gieson染色(以及其他需要的染色)。还提供了多种抗体的免疫组织化学染色。核心由一位有25年经验的全职历史学家(凯瑟琳·卡里尔饰)操作，他执行所有的日常操作。其中包括：组织处理(徕卡TP1020)和特殊染色；免疫组织化学；选择方案开发；共聚焦显微镜；以及请求处理、订购和核心维护。CORE主管(Volkhard Lindner)负责监督CORE的运作，并积极协助解释和评估组织标本。现场仪器包括：冷冻切片HM505E微型机；石蜡和塑料切片微型机HM355S和HM325微型机；用于石蜡渗透的Fisher组织中心包埋站、Lab-Line 3618型和VWR1410型真空炉；以及幻灯片加热器和水浴。为了进行集中的组织学操作，通过科布尔奖基金将一台自动组织处理机(徕卡TP1020)投入使用。2005年，一台自动玻片着色机(徕卡AUTOSTINER XL)和盖子滑移站(Hyperlean)上线。这个核心是在我们的干细胞科布雷中心很早就建立起来的，现在是高生产率(和成本产生)。就像B和D核心的情况一样，这个组织病理学核心无疑对推进我们新提出的项目和整体计划将是重要的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C provides expert histology services to Stem Cell COBRE, and additional MMCRI investigators. Services include tissue fixation, embedding, sectioning of frozen, paraffin or plastic embedded specimens, and certain staining procedures. Histological stains include Hematoxylin-Eosin, Trichrome, von Kossa, Orcein, van Gieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also provided. The Core is operated by a full time histologist with 25 years of experience (Kathleen Carrier) who performs all day-to-day operations. These include: tissue processing (Leica TP1020) and special stains; immunohistochemistry; select protocol development; confocal microscopy; and request processing, ordering and core maintenance. The Core director (Volkhard Lindner) oversees Core operations and actively assists in the interpretation and evaluation of histology specimens. In-place instrumentation includes: Microm HM505E Cryostat for frozen sections; Microm HM355S and Microm HM325 microtome for paraffin and plastic sections; Fisher HistoCenter embedding station, Lab-Line Model 3618 and VWR model 1410 vacuum ovens for paraffin infiltration; and slide warmers and water baths. For centralized histology operation, an automated tissue processor (Leica TP1020) was brought on line through COBRE award funds. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. This core was established early-on in our Stem Cell COBRE Center, and is now highly productive (and cost- generating). As is the case for cores B and D, this histopathology Core will no doubt be important for advancing our newly proposed projects, and overall Program.